Mutation in the P36 gene of Mycobacterium bovis provokes attenuation of the bacillus in a mouse model

P36 is a member of a family of secreted proteins distributed throughout the genus Mycobacterium. The central domain of these proteins contains several amino acid PGLTS repeats, which differ considerably between species. P36, also called exported repetitive protein (Erp) in M. tuberculosis, has been...

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Bibliographic Details
Published in:Tuberculosis (Edinburgh, Scotland) Scotland), 2005-07, Vol.85 (4), p.221-226
Main Authors: Bigi, F., Gioffré, A., Klepp, L., Santangelo, M.P., Velicovsky, C.A., Giambartolomei, G.H., Fossati, C.A., Romano, M.I., Mendum, T., McFadden, J.J., Cataldi, A.
Format: Article
Language:English
Subjects:
Animals
Attenuation
Bacillus
Bacillus - genetics
Bacterial Proteins - genetics
Blotting, Western
Electrophoresis, Polyacrylamide Gel
Female
Membrane Proteins - genetics
Mice
Mice, Inbred BALB C
Mutation - genetics
Mycobacteria
Mycobacterium bovis
Mycobacterium bovis - genetics
Mycobacterium tuberculosis
Virulence
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Summary:P36 is a member of a family of secreted proteins distributed throughout the genus Mycobacterium. The central domain of these proteins contains several amino acid PGLTS repeats, which differ considerably between species. P36, also called exported repetitive protein (Erp) in M. tuberculosis, has been shown to be associated with virulence since the disruption of its gene impaired multiplication of both virulent M. tuberculosis and M. bovis BCG in cultured macrophages and immunocompetent mice. In order to demonstrate that P36 is a putative virulence factor of wild-type Mycobacterium bovis we generated a P36 mutant by gene disruption and we evaluated its replication in spleen and lungs of infected mice. In this study, the mutant strain displays low levels of multiplication in mice, indicating that the P36 gene is important for in vivo growth of M. bovis.
ISSN:1472-9792
1873-281X
DOI:10.1016/j.tube.2004.11.002