Characterization and comparative analysis of Arabidopsis phosphatidylinositol phosphate 5-kinase 10 reveals differences in Arabidopsis and human phosphatidylinositol phosphate kinases

• Published in: FEBS letters 2005-06, Vol.579 (16), p.3427-3432
• Main Authors: Perera, Imara Y., Davis, Amanda J., Galanopoulou, Dia, Im, Yang Ju, Boss, Wendy F.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Arabidopsis; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - genetics; Arabidopsis Proteins - isolation & purification; Arabidopsis thaliana; glutathione-S-transferase; GST; Homo sapiens; Humans; Kinetics; Lipid kinase; membrane occupation and recognition nexus; Molecular Sequence Data; MORN; phosphatidic acid; phosphatidylinositol phosphate; Phosphatidylinositol phosphate kinase; Phosphatidylinositol Phosphates - chemistry; phosphatidylinositol-(4,5)-bisphosphate; phosphatidylinositol-3-phosphate; phosphatidylinositol-4-phosphate; phosphatidylinositol-5-phosphate; Phosphotransferases (Alcohol Group Acceptor) - chemistry; Phosphotransferases (Alcohol Group Acceptor) - genetics; Phosphotransferases (Alcohol Group Acceptor) - isolation & purification; Phosphotransferases (Alcohol Group Acceptor) - metabolism; PIPK; PtdIns(4,5)P2; PtdIns3P; PtdIns4P; PtdIns5P; PtdInsP; PtdOH; Sequence Alignment; Space life sciences; Substrate Specificity; Transcription, Genetic; β-cyclodextrin; βCD
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2005.05.018

Arabidopsis phosphatidylinositol phosphate (PtdInsP) kinase 10 ( AtPIPK10; At4g01190) is shown to be a functional enzyme of the subfamily A, type I AtPtdInsP kinases. It is biochemically distinct from AtPIPK1 (At1g21980), the only other previously characterized AtPtdInsP kinase which is of the B subfamily. AtPIPK10 has the same K m, but a 10-fold lower V max than AtPIPK1 and it is insensitive to phosphatidic acid. AtPIPK10 transcript is most abundant in inflorescence stalks and flowers, whereas AtPIPK1 transcript is present in all tissues. Comparative analysis of recombinant AtPIPK10 and AtPIPK1 with recombinant HsPIPKIα reveals that the Arabidopsis enzymes have roughly 200- and 20-fold lower V max/ K m, respectively. These data reveal one explanation for the longstanding mystery of the relatively low phosphatidylinositol-(4,5)-bisphosphate:phosphatidylinositol-4-phosphate ratio in terrestrial plants.