Quantitation of DNA of Polyomaviruses BK and JC in Human Kidneys

BackgroundRenal allograft recipients can be monitored for polyomavirus-associated nephropathy (PVAN) using urine samples. Because virus in urine can be derived from the kidneys, ureter, or urinary bladder, we evaluated whether measurement of intrarenal concentrations of viral DNA might serve as a mo...

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Bibliographic Details
Published in:The Journal of infectious diseases 2005-08, Vol.192 (3), p.504-509
Main Authors: Randhawa, Parmjeet, Shapiro, Ron, Vats, Abhay
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biopsies
BK virus
BK Virus - genetics
BK Virus - isolation & purification
BK Virus - physiology
DNA
DNA, Viral - genetics
DNA, Viral - isolation & purification
Fundamental and applied biological sciences. Psychology
Genome, Viral
Homologous transplantation
Humans
Infections
Infectious diseases
JC virus
JC Virus - genetics
JC Virus - isolation & purification
JC Virus - physiology
Kidney - pathology
Kidney - virology
Kidney Tubules - virology
Kidneys
Medical sciences
Microbiology
Plasmids
Polymerase Chain Reaction
Polyomavirus
Polyomavirus Infections - pathology
Simian virus 40 - genetics
Tissue samples
Tumor Virus Infections - pathology
Urine
Virus Latency
Viruses
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Summary:BackgroundRenal allograft recipients can be monitored for polyomavirus-associated nephropathy (PVAN) using urine samples. Because virus in urine can be derived from the kidneys, ureter, or urinary bladder, we evaluated whether measurement of intrarenal concentrations of viral DNA might serve as a more reliable monitoring tool MethodsReal-time quantitative polymerase chain reaction was used to quantitate DNA of polyomaviruses BK (BKV) and JC (JCV) in renal tissue obtained from various clinical settings ResultsRenal biopsy samples from 28 nonimmunosuppressed patients contained very low viral copy numbers. Minimally higher BKV loads (mean±SE, 3.4±1.7 copies/cell) were observed in 74 renal biopsy samples from renal allograft recipients with BKV viruria. The BKV DNA concentration was ∼10-fold higher in renal allograft recipients with BKV viruria, but 58 (50.4%) of 115 renal biopsy samples tested negative for BKV DNA, reflecting the focal nature of infection. JCV DNA was found in only 2 renal biopsy samples ConclusionsThe BKV load is better measured in urine than in tissue, because a urine sample represents material from the entire kidney. An increase in the BKV load is usually not accompanied by a proportional increase in the JCV load, which indicates that these 2 related polyomaviruses are subject to different mechanisms regulating viral replication
ISSN:0022-1899
1537-6613
DOI:10.1086/431522