In vitro interaction between surface-treated Ti-6Al-4V titanium alloy and human peripheral blood mononuclear cells

The Ti‐6Al‐4V titanium alloy is widely employed as an implant material. The effects of Ti‐6Al‐4V samples, tested in both an untreated state and one in which the samples were subjected to a glow‐discharge treatment performed with the use of air, on human peripheral blood mononuclear cells (PBMC) were...

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Bibliographic Details
Published in:Journal of biomedical materials research 2005-08, Vol.74A (2), p.197-207
Main Authors: Martinesi, Maria, Bruni, Sara, Stio, Maria, Treves, Cristina, Borgioli, Francesca
Format: Article
Language:English
Subjects:
Apoptosis
blood-material interaction
Blotting, Western
cell culture
Cells, Cultured
Coculture Techniques
Culture Media
cytokines
Endothelium, Vascular - cytology
Humans
Intercellular Adhesion Molecule-1 - metabolism
Monocytes - cytology
Monocytes - metabolism
surface modification
Surface Properties
titanium (alloys)
Titanium - chemistry
Tumor Necrosis Factor-alpha - metabolism
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Summary:The Ti‐6Al‐4V titanium alloy is widely employed as an implant material. The effects of Ti‐6Al‐4V samples, tested in both an untreated state and one in which the samples were subjected to a glow‐discharge treatment performed with the use of air, on human peripheral blood mononuclear cells (PBMC) were studied. Apoptosis, undetectable after 24‐h contact of PBMC with the two sample types, is induced after 48 h by treated samples, and, after 48 h, but in the presence of 1.5 μg/mL PHA, by both sample types. The expression of intercellular adhesion molecule‐1 (ICAM‐1) always increases, in comparison with control, in PBMC put in contact with the two sample types. In the same way, a remarkable increase in tumor necrosis‐alpha (TNF‐α) release in the culture medium is registered, when PBMC are put in contact with the two sample types for 24 and 48 h. Human umbilical‐vein endothelial cells (HUVEC) cocultured for 48 h with PBMC, previously incubated with the two sample types for 24 h, show an increase in ICAM‐1 and vascular cell adhesion molecule‐1 (VCAM‐1) protein expression in comparison with control (HUVEC cocultured with control PBMC), indicating that inflammatory phenomena might occur. Taken together, these results suggest that, although plasma‐treated titanium alloy shows a better biocompatibility in comparison with the untreated one, attention must be paid to the careful control of the first signs of inflammation. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005
ISSN:1549-3296
0021-9304
1552-4965
1097-4636
DOI:10.1002/jbm.a.30366