Divide and conquer: development and cell cycle genes in plant transformation

Genetic transformation and regeneration of transgenic plants remains unfeasible for the majority of plant species. We propose that inducible expression and/or suppression of the genes that control the cell cycle and development, by altering chromatin structure and exerting epigenetic control of gene...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2006-06, Vol.24 (6), p.267-273
Main Authors: Arias, Renée S., Filichkin, Sergei A., Strauss, Steven H.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
cell cycle
Cell Cycle - genetics
cell division
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant - genetics
Gene Silencing
Gene Transfer Techniques
genes
Genes, cdc
genetic transformation
genomics
Heterochromatin - genetics
literature reviews
molecular genetics
plant genetics
plants
Plants, Genetically Modified - cytology
Plants, Genetically Modified - genetics
temporal variation
Transformation, Genetic
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Summary:Genetic transformation and regeneration of transgenic plants remains unfeasible for the majority of plant species. We propose that inducible expression and/or suppression of the genes that control the cell cycle and development, by altering chromatin structure and exerting epigenetic control of gene expression, might substantially improve competence for transformation and/or regeneration. Transformation efficiency was higher in cells with nuclei at the S and G2 phases, and manipulating the genes whose activation or silencing promote the G1–S transition has increased both transient and stable transformation. Controlling the cell cycle directly, using RBR and VIP1, or indirectly, through hormone regulation using IPT and ESR1, has improved rates of stable transformation. Other target genes that might promote incorporation of DNA and/or pluripotency of cells include HP1, CycD3 and CycD1. The availability of large EST databanks, complete plant-genome sequences and/or inducible gene expression systems create opportunities for testing homologous genes to increase competence of transformation and regeneration.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2006.04.007