Functional expression of mammalian receptors and membrane channels in different cells

In native tissues, the majority of medically important membrane proteins is only present at low concentrations, making their overexpression in recombinant systems a prerequisite for structural studies. Here, we explore the commonly used eukaryotic expression systems—yeast, baculovirus/insect cells (...

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Bibliographic Details
Published in:Journal of structural biology 2007-08, Vol.159 (2), p.179-193
Main Authors: Eifler, Nora, Duckely, Myriam, Sumanovski, Lazar T., Egan, Terrance M., Oksche, Alexander, Konopka, James B., Lüthi, Anita, Engel, Andreas, Werten, Paul J.L.
Format: Article
Language:English
Subjects:
Animals
Cell Line
Eukaryotic expression systems
Gene Expression
Genetic Vectors
Humans
Membrane protein expression
Membrane Proteins - genetics
Membrane Proteins - metabolism
Patch-Clamp Techniques
Plasmids - genetics
Plasmids - metabolism
Protein activity
Protein localization
Protein solubility
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Solubility
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Summary:In native tissues, the majority of medically important membrane proteins is only present at low concentrations, making their overexpression in recombinant systems a prerequisite for structural studies. Here, we explore the commonly used eukaryotic expression systems—yeast, baculovirus/insect cells ( Sf9) and Semliki Forest Virus (SFV)/mammalian cells—for the expression of seven different eukaryotic membrane proteins from a variety of protein families. The expression levels, quality, biological activity, localization and solubility of all expressed proteins are compared in order to identify the advantages of one system over the other. SFV-transfected mammalian cell lines provide the closest to native environment for the expression of mammalian membrane proteins, and they exhibited the best overall performance. But depending on the protein, baculovirus-infected Sf9 cells performed almost as well as mammalian cells. The lowest expression levels for the proteins tested here were obtained in yeast.
ISSN:1047-8477
1095-8657
DOI:10.1016/j.jsb.2007.01.014