B Cell-Specific Deletion of Protein-Tyrosine Phosphatase Shp1 Promotes B-1a Cell Development and Causes Systemic Autoimmunity

Spontaneous loss-of-function mutations in the protein-tyrosine phosphatase Shp1 cause the motheaten phenotype, characterized by widespread inflammation and autoimmunity. Because Shp1 is expressed in all hematopoietic cells, it has been unclear which aspects of the motheaten phenotypes are primary ef...

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Bibliographic Details
Published in:Immunity (Cambridge, Mass.) Mass.), 2007-07, Vol.27 (1), p.35-48
Main Authors: Pao, Lily I., Lam, Kong-Peng, Henderson, Joel M., Kutok, Jeffery L., Alimzhanov, Marat, Nitschke, Lars, Thomas, Matthew L., Neel, Benjamin G., Rajewsky, Klaus
Format: Article
Language:English
Subjects:
Animals
Antigen-Antibody Complex - metabolism
Autoimmune Diseases - enzymology
Autoimmune Diseases - genetics
Autoimmune Diseases - pathology
B-Lymphocyte Subsets - cytology
B-Lymphocyte Subsets - enzymology
B-Lymphocyte Subsets - immunology
Bone marrow
Cell Differentiation - genetics
Cell Differentiation - immunology
Cells, Cultured
Cloning
Deoxyribonucleic acid
DNA
Gene Deletion
Genotype & phenotype
Glomerulonephritis - enzymology
Glomerulonephritis - genetics
Glomerulonephritis - immunology
Glomerulonephritis - pathology
Kinases
Mice
Mice, Inbred C57BL
Mice, Mutant Strains
MOLIMMUNO
Mutation
Protein Tyrosine Phosphatase, Non-Receptor Type 6 - deficiency
Protein Tyrosine Phosphatase, Non-Receptor Type 6 - genetics
Proteins
Rodents
Software
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Summary:Spontaneous loss-of-function mutations in the protein-tyrosine phosphatase Shp1 cause the motheaten phenotype, characterized by widespread inflammation and autoimmunity. Because Shp1 is expressed in all hematopoietic cells, it has been unclear which aspects of the motheaten phenotypes are primary effects of Shp1 deficiency. We generated mice ( Ptpn6 f/f; CD19 -cre) that delete Shp1 specifically in B cells. Analysis of these mice indicates that the increase in B-1a cells in motheaten mice is a cell-autonomous consequence of Shp1 deficiency. Shp1-deficient B-1a cells could be derived from adult bone marrow and had N-nucleotide additions, consistent with an adult origin. Shp1 deficiency altered calcium response evoked by B cell antigen receptors and impaired CD40-evoked proliferation. Young Ptpn6 f/f; CD19 -cre mice exhibited elevated serum immunoglobulins and impaired antibody responses to immunization, whereas older Ptpn6 f/f; CD19 -cre mice developed systemic autoimmunity, characterized by DNA antibodies and immune complex glomerulonephritis. Thus, Shp1 deficiency in B cells alone perturbs B cell development and causes autoimmune disease.
ISSN:1074-7613
1097-4180
DOI:10.1016/j.immuni.2007.04.016