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Microtubule-Severing Activity of Shigella Is Pivotal for Intercellular Spreading
Some pathogenic bacteria actually invade the cytoplasm of their target host cells. Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that Shigella movement within the cytoplasm was severely hindered by microtubules and that the b...
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| Published in: | Science (American Association for the Advancement of Science) 2006-11, Vol.314 (5801), p.985-989 |
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| Main Authors: | , , , , , , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c521t-870be096ca7cb960c1b7a237d1831f4e73eaec8f513a9050268a04cc99069bde3 |
| container_end_page | 989 |
| container_issue | 5801 |
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| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 314 |
| creator | Yoshida, Sei Handa, Yutaka Suzuki, Toshihiko Ogawa, Michinaga Suzuki, Masato Tamai, Asuka Abe, Akio Katayama, Eisaku Sasakawa, Chihiro |
| description | Some pathogenic bacteria actually invade the cytoplasm of their target host cells. Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that Shigella movement within the cytoplasm was severely hindered by microtubules and that the bacteria destroyed surrounding microtubules by secreting VirA by means of the type III secretion system. Degradation of microtubules by VirA was dependent on its α-tubulin-specific cysteine protease-like activity. virA mutants did not move within the host cytoplasm and failed to move into adjacent cells. |
| doi_str_mv | 10.1126/science.1133174 |
| format | article |
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Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that Shigella movement within the cytoplasm was severely hindered by microtubules and that the bacteria destroyed surrounding microtubules by secreting VirA by means of the type III secretion system. Degradation of microtubules by VirA was dependent on its α-tubulin-specific cysteine protease-like activity. virA mutants did not move within the host cytoplasm and failed to move into adjacent cells.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1133174</identifier><identifier>PMID: 17095701</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Actins ; Actins - metabolism ; Animals ; Antibodies ; Bacteria ; Bacteriology ; Biological and medical sciences ; Cell adhesion & migration ; Cercopithecus aethiops ; COS Cells ; Cysteine Endopeptidases - metabolism ; Cytoplasm ; Cytoplasm - microbiology ; Dysentery, Bacillary - microbiology ; Epithelial cells ; Fundamental and applied biological sciences. Psychology ; Immunoblotting ; Mice ; Mice, Inbred C57BL ; Microbiology ; Microscopy, Fluorescence ; Microtubules ; Microtubules - drug effects ; Microtubules - metabolism ; Microtubules - ultrastructure ; Movement ; Mutation ; Nocodazole - pharmacology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Polymerization ; Proteases ; Shigella ; Shigella flexneri - enzymology ; Shigella flexneri - genetics ; Shigella flexneri - pathogenicity ; Shigella flexneri - physiology ; Tubulin - metabolism ; Virulence Factors - metabolism</subject><ispartof>Science (American Association for the Advancement of Science), 2006-11, Vol.314 (5801), p.985-989</ispartof><rights>Copyright 2006 American Association for the Advancement of Science</rights><rights>2007 INIST-CNRS</rights><rights>Copyright American Association for the Advancement of Science Nov 10, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-870be096ca7cb960c1b7a237d1831f4e73eaec8f513a9050268a04cc99069bde3</citedby><cites>FETCH-LOGICAL-c521t-870be096ca7cb960c1b7a237d1831f4e73eaec8f513a9050268a04cc99069bde3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2884,2885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18294311$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17095701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshida, Sei</creatorcontrib><creatorcontrib>Handa, Yutaka</creatorcontrib><creatorcontrib>Suzuki, Toshihiko</creatorcontrib><creatorcontrib>Ogawa, Michinaga</creatorcontrib><creatorcontrib>Suzuki, Masato</creatorcontrib><creatorcontrib>Tamai, Asuka</creatorcontrib><creatorcontrib>Abe, Akio</creatorcontrib><creatorcontrib>Katayama, Eisaku</creatorcontrib><creatorcontrib>Sasakawa, Chihiro</creatorcontrib><title>Microtubule-Severing Activity of Shigella Is Pivotal for Intercellular Spreading</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Some pathogenic bacteria actually invade the cytoplasm of their target host cells. Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that Shigella movement within the cytoplasm was severely hindered by microtubules and that the bacteria destroyed surrounding microtubules by secreting VirA by means of the type III secretion system. Degradation of microtubules by VirA was dependent on its α-tubulin-specific cysteine protease-like activity. virA mutants did not move within the host cytoplasm and failed to move into adjacent cells.</description><subject>Actins</subject><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell adhesion & migration</subject><subject>Cercopithecus aethiops</subject><subject>COS Cells</subject><subject>Cysteine Endopeptidases - metabolism</subject><subject>Cytoplasm</subject><subject>Cytoplasm - microbiology</subject><subject>Dysentery, Bacillary - microbiology</subject><subject>Epithelial cells</subject><subject>Fundamental and applied biological sciences. 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Invasive bacteria acquire the propulsive force to move by recruiting actin and inducing its polymerization. Here we show that Shigella movement within the cytoplasm was severely hindered by microtubules and that the bacteria destroyed surrounding microtubules by secreting VirA by means of the type III secretion system. Degradation of microtubules by VirA was dependent on its α-tubulin-specific cysteine protease-like activity. virA mutants did not move within the host cytoplasm and failed to move into adjacent cells.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>17095701</pmid><doi>10.1126/science.1133174</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 2006-11, Vol.314 (5801), p.985-989 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_68134519 |
| source | American Association for the Advancement of Science; Alma/SFX Local Collection |
| subjects | Actins Actins - metabolism Animals Antibodies Bacteria Bacteriology Biological and medical sciences Cell adhesion & migration Cercopithecus aethiops COS Cells Cysteine Endopeptidases - metabolism Cytoplasm Cytoplasm - microbiology Dysentery, Bacillary - microbiology Epithelial cells Fundamental and applied biological sciences. Psychology Immunoblotting Mice Mice, Inbred C57BL Microbiology Microscopy, Fluorescence Microtubules Microtubules - drug effects Microtubules - metabolism Microtubules - ultrastructure Movement Mutation Nocodazole - pharmacology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Polymerization Proteases Shigella Shigella flexneri - enzymology Shigella flexneri - genetics Shigella flexneri - pathogenicity Shigella flexneri - physiology Tubulin - metabolism Virulence Factors - metabolism |
| title | Microtubule-Severing Activity of Shigella Is Pivotal for Intercellular Spreading |
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