Involvement of KC, MIP-2, and MCP-1 in leukocyte infiltration following injection of necrotic cells into the peritoneal cavity

Although necrotic cells are known to induce inflammation in vivo, the underlying mechanism remains largely unexplored. In order to examine the mechanism, we used an inflammation model induced by injection of necrotic leukemic P388 cells into the peritoneal cavity in this study. The injection of necr...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-09, Vol.361 (2), p.533-536
Main Authors: Tanimoto, Nahoko, Terasawa, Masao, Nakamura, Miho, Kegai, Daisuke, Aoshima, Naho, Kobayashi, Yoshiro, Nagata, Kisaburo
Format: Article
Language:English
Subjects:
Animals
Antibodies
Cell infiltration
Chemokine
Chemokine CCL2 - metabolism
Chemokine CXCL1
Chemokine CXCL2
Chemokines, CXC - metabolism
Injections
Male
MCP-1
Mice
MIP-2
Monokines - metabolism
Necrosis - pathology
Necrotic cells
Neutralization Tests
Neutrophil Infiltration
Peritoneal Cavity - pathology
Receptors, Chemokine - metabolism
Time Factors
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Summary:Although necrotic cells are known to induce inflammation in vivo, the underlying mechanism remains largely unexplored. In order to examine the mechanism, we used an inflammation model induced by injection of necrotic leukemic P388 cells into the peritoneal cavity in this study. The injection of necrotic cells induced the infiltration of neutrophils and subsequently that of monocytes/macrophages. In agreement with this, the injection also induced the production of KC and MIP-2, and subsequently that of MCP-1. Although the level of KC was higher than that of MIP-2, both anti-KC Ab and anti-MIP-2 Ab significantly inhibited the infiltration of neutrophils. Antibodies against CXCR2, a sole receptor for KC and MIP-2, almost completely inhibited the infiltration of neutrophils and monocytes/macrophages. Anti-MCP-1 Ab, on the other hand, inhibited the infiltration of monocytes/macrophages but not neutrophils. These results indicate that KC and MIP-2 play important roles in the infiltration of neutrophils into the site of injection of necrotic cells and that neutrophils may regulate monocyte/macrophage infiltration in our model.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.07.060