A Novel Function of Ionotropic γ-Aminobutyric Acid Receptors Involving Alveolar Fluid Homeostasis

Polarized distribution of chloride channels on the plasma membrane of epithelial cells is required for fluid transport across the epithelium of fluid-transporting organs. Ionotropic γ-aminobutyric acid receptors are primary ligand-gated chloride channels that mediate inhibitory neurotransmission. Tr...

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Bibliographic Details
Published in:The Journal of biological chemistry 2006-11, Vol.281 (47), p.36012-36020
Main Authors: Jin, Nili, Kolliputi, Narasiah, Gou, Deming, Weng, Tingting, Liu, Lin
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Animals
Biotinylation
Cell Membrane - metabolism
Chloride Channels - chemistry
Chlorides - chemistry
Immunoprecipitation
Ligands
Lung - metabolism
Pulmonary Alveoli - metabolism
Rats
Receptors, GABA - metabolism
Receptors, GABA - physiology
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference
Time Factors
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Summary:Polarized distribution of chloride channels on the plasma membrane of epithelial cells is required for fluid transport across the epithelium of fluid-transporting organs. Ionotropic γ-aminobutyric acid receptors are primary ligand-gated chloride channels that mediate inhibitory neurotransmission. Traditionally, these receptors are not considered to be contributors to fluid transport. Here, we report a novel function of γ-aminobutyric acid receptors involving alveolar fluid homeostasis in adult lungs. We demonstrated the expression of functional ionotropic γ-aminobutyric acid receptors on the apical plasma membrane of alveolar epithelial type II cells. γ-Aminobutyric acid significantly increased chloride efflux in the isolated type II cells and inhibited apical to basolateral chloride transport on type II cell monolayers. Reduction of the γ-aminobutyric acid receptor π subunit using RNA interference abolished the γ-aminobutyric acid-mediated chloride transport. In intact rat lungs, γ-aminobutyric acid inhibited both basal and β agonist-stimulated alveolar fluid clearance. Thus, we provide molecular and pharmacological evidence that ionotropic γ-aminobutyric acid receptors contribute to fluid transport in the lung via luminal secretion of chloride. This finding may have the potential to develop clinical approaches for pulmonary diseases involving abnormal fluid dynamics.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M606895200