Ultrastructural alterations induced by the neolignan dihydrobenzofuranic eupomatenoid-5 on epimastigote and amastigote forms of Trypanosoma cruzi

Eupomatenoid-5, a compound isolated from leaves of Piper regnellii var. pallescens, showed antiprotozoal activity against the epimastigote proliferative stages and intracellular amastigote forms of Trypanosoma cruzi Y strain. Eupomatenoid-5, at 7.0 microg/ml (50% growth inhibition concentration) pro...

Full description

Saved in:
Bibliographic Details
Published in:Parasitology research (1987) 2006-12, Vol.100 (1), p.31-37
Main Authors: SHIMA LUIZE, Patricia, UEDA-NAKAMURA, Tania, PRADO DIAS FILHO, Benedito, APARICIO GARCIA CORTEZ, Diogenes, MORGADO-DIAZ, José Andrés, DE SOUZA, Wanderley, VATARU NAKAMURA, Celso
Format: Article
Language:English
Subjects:
Animals
Benzofurans - pharmacology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Invertebrates
Microscopy, Electron, Scanning
Phenols - pharmacology
Piper
Plant Leaves
Trypanocidal Agents - pharmacology
Trypanosoma cruzi - drug effects
Trypanosoma cruzi - growth & development
Trypanosoma cruzi - ultrastructure
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Eupomatenoid-5, a compound isolated from leaves of Piper regnellii var. pallescens, showed antiprotozoal activity against the epimastigote proliferative stages and intracellular amastigote forms of Trypanosoma cruzi Y strain. Eupomatenoid-5, at 7.0 microg/ml (50% growth inhibition concentration) produced morphological changes in epimastigote forms of the parasite, such as intense cytoplasmic vacuolization, mitochondrial swelling, kinetoplast alteration, presence of myelin-like figures, and mitochondrial damage, as observed by transmission electron microscopy. In amastigote forms in LLCMK(2) cells, at 7.0-microg/ml concentration, the compound induced a decrease in the number of cells with internalized parasites and in the number of internalized parasites per LLCMK(2) cell, compared with untreated cells. Furthermore, intense cytoplasmic vacuolization and autophagic vacuoles were observed in T. cruzi intracellular amastigotes after 72 h of incubation. Scanning electron microscopy confirmed the alterations in the shape of the parasites.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-006-0241-9