Regulated Autophagy Controls Hormone Content in Secretory-Deficient Pancreatic Endocrine β-Cells

• Published in: Molecular endocrinology (Baltimore, Md.) Md.), 2007-09, Vol.21 (9), p.2255-2269
• Main Authors: Marsh, Brad J, Soden, Chad, Alarcón, Cristina, Wicksteed, Barton L, Yaekura, Kazuro, Costin, Adam J, Morgan, Garry P, Rhodes, Christopher J
• Format: Article
• Language: English
• Subjects: Animals; Autophagy - physiology; Insulin - metabolism; Insulin Secretion; Insulin-Secreting Cells - metabolism; Insulin-Secreting Cells - pathology; Mice; Mice, Inbred C57BL; Mice, Knockout; Proinsulin - biosynthesis; rab3A GTP-Binding Protein - deficiency; rab3A GTP-Binding Protein - genetics
• ISSN: 0888-8809; 1944-9917
• DOI: 10.1210/me.2007-0077

Endocrine cells are continually regulating the balance between hormone biosynthesis, secretion, and intracellular degradation to ensure that cellular hormone stores are maintained at optimal levels. In pancreatic β-cells, intracellular insulin stores in β-granules are mostly upheld by efficiently up-regulating proinsulin biosynthesis at the translational level to rapidly replenish the insulin lost via exocytosis. Under normal circumstances, intracellular degradation of insulin plays a relatively minor janitorial role in retiring aged β-granules, apparently via crinophagy. However, this mechanism alone is not sufficient to maintain optimal insulin storage in β-cells when insulin secretion is dysfunctional. Here, we show that despite an abnormal imbalance of glucose/glucagon-like peptide 1 regulated insulin production over secretion in Rab3A−/− mice compared with control animals, insulin storage levels were maintained due to increased intracellular β-granule degradation. Electron microscopy analysis indicated that this was mediated by a significant 12-fold up-regulation of multigranular degradation vacuoles in Rab3A−/− mouse islet β-cells (P ≤ 0.001), which by further electron microscopy-tomography analysis was found to be mostly contributed by microautophagic activity. This increased autophagic activity in Rab3A−/− mouse islet β-cells was associated with a specific decrease in islet lysosomal-associated membrane protein 2 gene expression (P ≤ 0.05), at both the mRNA and protein expression levels. Lysosomal-associated membrane protein 2 is a documented negative regulator of autophagy. These findings indicate that the up-regulation of degradative pathways provides secretory-deficient endocrine cells with a compensatory mechanism for regulating their intracellular hormone content in vivo. These data may also have implications for the β-cell’s response to diminished insulin secretion during the pathogenesis of type 2 diabetes.