The giant fibrillar center: A nucleolar structure enriched in upstream binding factor (UBF) that appears in transcriptionally more active sensory ganglia neurons

• Published in: Journal of structural biology 2007-09, Vol.159 (3), p.451-461
• Main Authors: Casafont, Iñigo, Bengoechea, Rocio, Navascués, Joaquín, Pena, Emma, Berciano, Maria T., Lafarga, Miguel
• Format: Article
• Language: English
• Subjects: 5′-Fluorouridine transcription assay; Animals; Cajal bodies; Cell Differentiation; Cell Nucleolus - chemistry; Cell Nucleolus - ultrastructure; Ganglia, Sensory - growth & development; Ganglia, Sensory - metabolism; Ganglia, Sensory - ultrastructure; Giant fibrillar centers; Male; Microscopy, Immunoelectron; Neuronal differentiation; Neurons, Afferent - metabolism; Neurons, Afferent - ultrastructure; Nucleolus; Pol1 Transcription Initiation Complex Proteins - analysis; Proteasome Endopeptidase Complex - analysis; Proteasome Endopeptidase Complex - metabolism; Rats; Rats, Sprague-Dawley; RNA, Ribosomal - analysis; RNA, Ribosomal - metabolism; Sensory ganglia neurons; SUMO-1; SUMO-1 Protein - analysis; SUMO-1 Protein - metabolism; Transcription, Genetic; UBF; Ubiquitin - analysis; Ubiquitin - metabolism; Ubiquitin–proteasome system
• ISSN: 1047-8477; 1095-8657
• DOI: 10.1016/j.jsb.2007.05.004

This paper studies the molecular organization, neuronal distribution and cellular differentiation dynamics of the giant fibrillar centers (GFCs) of nucleoli in rat sensory ganglia neurons. The GFC appeared as a round nucleolar domain (1–2 μm in diameter) partially surrounded by the dense fibrillar component and accompanied by numerous small FCs. By immunocytochemistry, the GFC concentrated the upstream binding factor, which may serve as a marker of this structure, and also contain RNA polymerase I, DNA topoisomerase I, SUMO-1 and Ubc9. However, they lack ubiquitin–proteasome conjugates and 20S proteasome. Transcription assay with 5′-fluorouridine incorporation revealed the presence of nascent RNA on the dense fibrillar component of the neuronal nucleolus, but not within the low electron-density area of the GFC. The formation of GFCs is neuronal size dependent: they were found in 58%, 30% and 0% of the large, medium and small neurons, respectively. GFCs first appeared during the postnatal period, concomitantly with a stage of neuronal growth, myelination and bioelectrical maturation. GFCs were not observed in segregated nucleoli induced by severe inhibition of RNA synthesis. We suggest that the formation of GFCs is associated with a high rate of ribosome biogenesis of the transcriptionally more active large-size neurons.