Comparative Evaluation of Three Lignin Isolation Protocols for Various Wood Species

Milled wood lignin (MWL), cellulolytic enzyme lignin (CEL), and enzymatic mild acidolysis lignin (EMAL) were isolated from different wood species and characterized by various techniques. The EMAL protocol offered gravimetric lignin yields 2−5 times greater than those of the corresponding MWL and CEL...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2006-12, Vol.54 (26), p.9696-9705
Main Authors: Guerra, Anderson, Filpponen, Ilari, Lucia, Lucian A, Argyropoulos, Dimitris S
Format: Article
Language:English
Subjects:
Abies - chemistry
Abies concolor
acid treatment
ball milling
Biological and medical sciences
cellulases
cellulolytic enzyme lignin
chemical structure
enzymatic mild acidolysis lignin
enzymatic treatment
Eucalyptus - chemistry
Eucalyptus globulus
extraction
Food industries
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
isolation
lignin
Lignin - chemistry
Lignin - isolation & purification
Magnetic Resonance Spectroscopy
milled wood lignin
milling
Molecular Weight
nuclear magnetic resonance spectroscopy
Pinus - chemistry
Pinus palustris
Pseudotsuga - chemistry
Pseudotsuga menziesii
Sequoia - chemistry
Sequoia sempervirens
Species Specificity
wood
Wood - chemistry
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Summary:Milled wood lignin (MWL), cellulolytic enzyme lignin (CEL), and enzymatic mild acidolysis lignin (EMAL) were isolated from different wood species and characterized by various techniques. The EMAL protocol offered gravimetric lignin yields 2−5 times greater than those of the corresponding MWL and CEL. The purities of the EMALs were 3.75−10.6% higher than those of their corresponding CELs, depending upon the wood species from which they were isolated. Molecular weight analyses showed that the EMAL protocol isolates lignin fractions that are not accessed by the other procedures evaluated, while 31P NMR spectroscopy revealed that MWL is more condensed and bears more phenolic hydroxyl groups than EMAL and CEL. The yields and purities of EMAL, MWL, and CEL from hardwood were greater than those obtained for the examined softwoods. Structural details obtained by DFRC (derivatization followed by reductive cleavage)/31P NMR revealed different contents of condensed and uncondensed β-O-aryl ether structures, dibenzodioxocins, and condensed and uncondensed phenolic hydroxyl and carboxylic acid groups within lignins isolated from different wood species. Keywords: EMAL; MWL; CEL; DFRC; 31P NMR; ball milling; lignin; southern pine; Douglas fir; white fir; redwood; Eucalyptus globulus; compression wood
ISSN:0021-8561
1520-5118
DOI:10.1021/jf062433c