Controlling DNA Capture and Propagation through Artificial Nanopores

Electrophorescing biopolymers across nanopores modulates the ionic current through the pore, revealing the polymer's diameter, length, and conformation. The rapidity of polymer translocation (∼30 000 bp/ms) in this geometry greatly limits the information that can be obtained for each base. Here...

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Bibliographic Details
Published in:Nano letters 2007-09, Vol.7 (9), p.2824-2830
Main Authors: Trepagnier, Eliane H, Radenovic, Aleksandra, Sivak, David, Geissler, Phillip, Liphardt, Jan
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Condensed matter: structure, mechanical and thermal properties
Diffusion
DNA - chemistry
DNA - radiation effects
Electromagnetic Fields
Electroporation - methods
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties
Membranes, Artificial
Methods. Procedures. Technologies
Motion
Nanostructures - chemistry
Nanostructures - radiation effects
Optical Tweezers
Others
Physics
Porosity
Surfaces and interfaces
thin films and whiskers (structure and nonelectronic properties)
Various methods and equipments
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Summary:Electrophorescing biopolymers across nanopores modulates the ionic current through the pore, revealing the polymer's diameter, length, and conformation. The rapidity of polymer translocation (∼30 000 bp/ms) in this geometry greatly limits the information that can be obtained for each base. Here we show that the translocation speed of λ-DNA through artificial nanopores can be reduced using optical tweezers. DNAs coupled to optically trapped beads were presented to nanopores. DNAs initially placed up to several micrometers from the pore could be captured. Subsequently, the optical tweezers reduced translocation speeds to 150 bp/ms, about 200-fold slower than free DNA. Moreover, the optical tweezers allowed us to “floss” single polymers back and forth through the pore. The combination of controlled sample presentation, greatly slowed translocation speeds, and repeated electrophoresis of single DNAs removes several barriers to using artificial nanopores for sequencing, haplotyping, and characterization of protein−DNA interactions.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl0714334