Polymer brush-stabilized polyplex for a siRNA carrier with long circulatory half-life

• Published in: Journal of controlled release 2007-10, Vol.122 (3), p.209-216
• Main Authors: Sato, Ayumi, Choi, Sung Won, Hirai, Miwa, Yamayoshi, Asako, Moriyama, Rui, Yamano, Takeshi, Takagi, Motoki, Kano, Arihiro, Shimamoto, Akira, Maruyama, Atsushi
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blood circulation; Dextrans - chemistry; Drug Carriers - chemistry; Drug Stability; General pharmacology; Graft copolymer; Half-Life; Injections, Intravenous; Male; Medical sciences; Mice; Mice, Inbred ICR; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polyethylene Glycols - chemistry; Polylysine - analogs & derivatives; Polylysine - chemistry; Polymer brush; Polyplex; RNA, Small Interfering - administration & dosage; RNA, Small Interfering - blood; RNA, Small Interfering - pharmacokinetics; siRNA; Solubility
• ISSN: 0168-3659; 1873-4995
• DOI: 10.1016/j.jconrel.2007.04.018

Delivery systems of small interfering RNA (siRNA) are the key to siRNA therapeutic application. In this study, we prepared and evaluated a series of cationic comb-type copolymers (CCCs) possessing a polycationic backbone (less than 30 weight (wt) %) and abundant water-soluble side chains (more than 70 wt.%) as a siRNA carrier with prolonged blood circulation time. Markedly, the CCC with the higher side chain content (10 wt.% PLL and 90 wt.% PEG) showed stronger interaction with siRNA than that with the lower content (30 wt.% PLL and 70 wt.% PEG), suggesting that highly dense PEG brush reinforces interpolyelectrolyte complex between the PLL backbone and siRNA. The siRNA complexed with the CCC was resistant to nucleases in 90% plasma for 24 h in vitro. The CCC having the higher side chain content increased circulation time of siRNA in mouse bloodstream by 100-fold. Surprisingly, even when the CCC and siRNA were separately injected into mouse at 20 min interval, blood circulation of post-injected siRNA was significantly increased. These results imply that the CCC has higher selectivity in its ionic interaction with siRNA than other anionic substances in blood stream. To our knowledge, this is the first example of a polyplex carrier that prolongs blood circulation time of unmodified siRNA without resource-consuming preparation process.