Salivary isoprostanes indicate increased oxidation injury in periodontitis with additional tobacco abuse

• Published in: BioFactors (Oxford) 2006, Vol.28 (1), p.21-31
• Main Authors: Wolfram, Roswitha M., Budinsky, Alexandra C., Eder, Andreas, Presenhuber, Christiane, Nell, Andrea, Sperr, Wolfgang, Sinzinger, Helmut
• Format: Article
• Language: English
• Subjects: 6-Ketoprostaglandin F1 alpha - metabolism; Adult; Aged; Dinoprost - analogs & derivatives; Dinoprost - metabolism; Female; Humans; Isoprostanes - analysis; Isoprostanes - metabolism; Male; Middle Aged; Multivariate Analysis; Oxidation-Reduction; Oxidative Stress; periodontital disease; Periodontitis - metabolism; Saliva - chemistry; salivary isoprostanes; Smoking; Thromboxane B2 - metabolism
• ISSN: 0951-6433; 1872-8081
• DOI: 10.1002/biof.5520280103

Isoprostanes (IPs) are indicators of in‐vivo oxidative stress, and have been successfully used as markers for chronic inflammatory processes. The presence of chronic periodontal disease and cigarette smoking has been individually linked to the development of atherosclerosis, yet data regarding oxidative stress in this context are not available yet. The aim of this study was to evaluate levels of the salivary prostaglandins (PGs) 8‐epi‐PGF2α, 6‐oxo‐PGF1α, thromboxane B2 (TXB2) and PGF2α in association with periodontal disease status with and without additional cigarette smoking. We analyzed saliva samples from 121 adults, (aged 21–73 years, 90 non‐smokers, 31 smokers) for levels of 8‐epi‐PGF2α, 6‐oxo‐PGF1α, TXB2 and PGF2α. On the basis of periodontal disease indices the periodontal status of each subject was assessed and outcomes were then correlated with smoking status and laboratory findings. Salivary 8‐epi‐PGF2α levels increased with deteriorating plaque index, and were significantly higher (115.5 ± 23.5 pg/ml) in smoking individuals, when compared to non‐smokers (70.2 ± 20.4 pg/ml, p