PHARMACOKINETICS OF FLORFENICOL AFTER A SINGLE INTRAMUSCULAR DOSE IN WHITE-SPOTTED BAMBOO SHARKS (CHILOSCYLLIUM PLAGIOSUM)

• Published in: Journal of zoo and wildlife medicine 2006-06, Vol.37 (2), p.165-173
• Main Authors: Zimmerman, Dawn M, Armstrong, Douglas L, Curro, Thomas G, Dankoff, Sarah M, Vires, Kathleen W, Cook, Kimberly K, Jaros, Nathan D, Papich, Mark G
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - blood; Anti-Bacterial Agents - cerebrospinal fluid; Anti-Bacterial Agents - pharmacokinetics; Area Under Curve; Bamboo shark; Bamboos; Blood; Blood plasma; Chiloscyllium plagiosum; Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - veterinary; Dosage; Dose-Response Relationship, Drug; Female; florfenicol; Half lives; Half-Life; Injections, Intramuscular - veterinary; Male; Marine; meningitis; Meningitis, Bacterial - drug therapy; Meningitis, Bacterial - veterinary; Microbial Sensitivity Tests - veterinary; Pharmacokinetics; Pilot Projects; Sharks; Sharks - blood; Sharks - metabolism; Thiamphenicol - analogs & derivatives; Thiamphenicol - blood; Thiamphenicol - cerebrospinal fluid; Thiamphenicol - pharmacokinetics; Vibrio; Zoos
• ISSN: 1042-7260; 1937-2825
• DOI: 10.1638/05-065.1

This study evaluated the pharmacokinetics of florfenicol in the white-spotted bamboo shark (Chiloscyllium plagiosum). In addition to the pharmacokinetics, the potential application for treatment of bacterial meningitis was explored. A pilot study was used to compare doses of 30, 40, and 50 mg/kg i.m. Following that study, 10 adult sharks were administered a single i.m. dose of florfenicol at 40 mg/kg. Plasma and cerebrospinal fluid were collected and analyzed for florfenicol by a sensitive and specific high-pressure liquid chromatographic method. Pharmacokinetic analysis was performed using both non-compartmental and compartmental techniques. The absorption produced an average peak at 54 (±19) hr from the i.m. site of administration, and the half-life was prolonged, averaging 269.79 hr (±135.87). Florfenicol plasma concentrations peaked at an average of 11.85 μg/ml (±1.45) and were maintained above our target minimum inhibitory concentration of 4–8 μg/ml for at least 120 hr. Cerebrospinal fluid concentrations peaked at an estimated 9 μg/ml around 48 hr, surpassing the target minimum inhibitory concentration for at least 72 hr.