Decreased sensitivity of transformed 3T3-SV40 cells treated with N-acetylcysteine to bacterial invasion

Long-term treatment of transformed 3T3-SV40 mouse fibroblasts with antioxidant N-acetylcysteine decreased cell level of ROS and increased the concentration of reduced glutathione. Removal of N-acetylcysteine from the medium led to the appearance of well-expressed stress fibrils, virtually absent in...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2006-07, Vol.142 (1), p.90-93
Main Authors: Gamalei, I A, Efremova, T N, Kirpichnikova, K M, Komissarchik, Ya Yu, Kever, L V, Polozov, Yu V, Khaitlina, S Yu
Format: Article
Language:English
Subjects:
Acetylcysteine
Acetylcysteine - pharmacology
Actin
Actins - metabolism
Animals
Antioxidants
BALB 3T3 Cells
Cell Line, Transformed
Cell Transformation, Viral - drug effects
Cell Transformation, Viral - physiology
Endopeptidases - metabolism
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - metabolism
Fibrils
Fibroblasts
Glutathione
Glutathione - metabolism
Mice
Microscopy, Electron
Microscopy, Fluorescence
Phenotypes
Reactive Oxygen Species - metabolism
Reversion
Simian virus 40
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Summary:Long-term treatment of transformed 3T3-SV40 mouse fibroblasts with antioxidant N-acetylcysteine decreased cell level of ROS and increased the concentration of reduced glutathione. Removal of N-acetylcysteine from the medium led to the appearance of well-expressed stress fibrils, virtually absent in control cells. In contrast to control cells, these cells were not invaded by apathogenic Escherichia coli A2 strain producing ECP32 protease specifically cleaving actin. Antioxidant N-acetylcysteine can cause partial reversion of transformed phenotype at the expense of a shift of cell redox balance in favor of reduced glutathione.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-006-0300-3