Occurrence of human intestinal spirochetosis in comparison with infections by other enteropathogenic agents in an area of the Northern Italy

• Published in: Diagnostic microbiology and infectious disease 2007-10, Vol.59 (2), p.157-163
• Main Authors: Calderaro, Adriana, Gorrini, Chiara, Peruzzi, Simona, Piccolo, Giovanna, Dettori, Giuseppe, Chezzi, Carlo
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Biological and medical sciences; Brachyspira; Brachyspira - classification; Brachyspira - genetics; Brachyspira - isolation & purification; Brachyspira aalborgi; Brachyspira pilosicoli; Child; Child, Preschool; DNA, Bacterial - analysis; Epidemiology; Feces - microbiology; Feces - virology; Female; Genes, rRNA; Gram-Negative Bacterial Infections - epidemiology; Gram-Negative Bacterial Infections - microbiology; Human bacterial diseases; Human intestinal spirochetosis; Humans; Infant; Infectious Disease; Infectious diseases; Internal Medicine; Intestinal Diseases - epidemiology; Intestinal Diseases - microbiology; Intestinal Diseases - virology; Italy - epidemiology; Male; Medical sciences; Middle Aged; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 16S - genetics
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2007.05.002

Abstract The aim of this study was to investigate the occurrence of human intestinal spirochetosis (IS) by a 16S rRNA restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) in a selected group (234) of patients with gastrointestinal complaints and/or potential risk factors for IS in comparison with the occurrence of infections by other enteropathogenic agents. By using 16S rRNA RFLP-PCR, 16 patients (6.8%) with IS were found (11 infected by Brachyspira aalborgi , 3 by Brachyspira pilosicoli , and 2 by both species); moreover, 10 patients with gastroenteric viruses (4.2%), 13 with enteropathogenic bacteria other than intestinal spirochetes (5.5%), and 24 with intestinal parasites (10.2%) were found. This study provides an enhancement of the knowledge about the distribution of IS, suggesting that it may be more frequent than suspected and that clinicians should consider IS when patients present with long-standing diarrhea. Moreover, 16S rRNA RFLP-PCR might be a powerful tool not only for diagnostic purpose but also to investigate the occurrence of IS just on fecal samples, not requiring invasive diagnostic techniques.