In vivo real-time vessel imaging and ex vivo 3D reconstruction of atherosclerotic plaque in apolipoprotein E-knockout mice using synchrotron radiation microscopy

Abstract Background To examine the pathophysiology of atherosclerosis, imaging the vascular wall and pathology without tissue damage is required. We used the unmonochromatized synchrotron X-ray to acquire in vivo real-time and ex vivo images of atherosclerotic lesions in apo E-knockout mice without...

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Bibliographic Details
Published in:International journal of cardiology 2007-01, Vol.114 (2), p.166-171
Main Authors: Kim, Jin Won, Seo, Hong Seog, Hwu, Y, Je, Jung Ho, Kim, Aeree, Oh, Chilh Wan, Suh, Soon Yong, Rha, Seung Woon, Park, Chang Gyu, Oh, Dong Joo
Format: Article
Language:English
Subjects:
Animals
Aortic Diseases - pathology
Apo E-knockout mouse
Apolipoproteins E - genetics
Atherosclerosis
Atherosclerosis (general aspects, experimental research)
Atherosclerosis - pathology
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Cardiovascular
Imaging, Three-Dimensional
Male
Medical sciences
Mice
Mice, Knockout
Plaque
Synchrotron
Synchrotrons
Unmonochromatized
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Summary:Abstract Background To examine the pathophysiology of atherosclerosis, imaging the vascular wall and pathology without tissue damage is required. We used the unmonochromatized synchrotron X-ray to acquire in vivo real-time and ex vivo images of atherosclerotic lesions in apo E-knockout mice without contrast agents or staining. Methods In the five apo E-knockout mice (apo E−/−, 12, 24, 32, 48, 62-week-old, 3 males) and age/sex matched five wild type mice on cow diets, we acquired in vivo real-time images of thoracic aorta without contrast agents and then, the central arterial trees were dissected intact. Ex vivo synchrotron images with tomographic reconstruction were done and compared with the corresponding pathology. Results For all living animals, in vivo real-time images of thoracic aorta could be acquired without contrast agents but could not identify the atherosclerotic lesions. Ex vivo images accurately determined aortic wall and atherosclerotic plaque without staining in comparison to histopathology according to the AHA classification ( r = 0.84, p < 0.001). The volume rendered 3 D images of plaque showed central cholesterol clefts as matched with optical images. Conclusions The combination of synchrotron enhanced X-ray microscopy and genetically engineered hyperlipidemic animals would be a useful tool to investigate the changes of advanced atherosclerotic lesions.
ISSN:0167-5273
1874-1754
DOI:10.1016/j.ijcard.2005.12.010