Optimization of native agar extraction from Hydropuntia cornea from Yucatán, México

• Published in: Bioresource technology 2007-04, Vol.98 (6), p.1278-1284
• Main Authors: Pereira-Pacheco, F., Robledo, D., Rodríguez-Carvajal, L., Freile-Pelegrín, Y.
• Format: Article
• Language: English
• Subjects: Agar; Agar - chemistry; Agar - isolation & purification; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Hydropuntia; Hydropuntia cornea; Mexico; Native agar; Optimization; Rhodophyta - chemistry; Temperature; Viscosity
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2006.05.016

Response surface methodology (RSM) was used to determine optimum extraction conditions for yield, gelling temperature, melting temperature and apparent viscosity of native agar from the red alga Hydropuntia cornea. Two independent variables were selected during extraction, temperature (80, 90 and 100 °C) and time (2, 3 and 4 h). The extraction procedure using 100 °C and 3 h produced the greatest yield (43.3%), the greatest gelling temperature (32 °C) and the greatest melting temperature (78 °C). The extraction using 65 °C and 4 h produced the optimum statistical apparent viscosity (80.73 cPs), though agar yield under these conditions had low quality for industrial purposes. Temperature and time do not affect melting temperature, but do play an important role in gelling temperature. From the independent variables studied, extraction temperature is most important in producing an increase in yield, and time is the only variable that contributes significantly to increasing viscosity. Most of the physicochemical properties found in the native agar from H. cornea extracted at 100 °C/3 h were similar to commercial samples. These findings suggest the feasibility of using this colloid in spreads and soft-texture food products.