Pannexin1 is expressed by neurons and glia but does not form functional gap junctions

Pannexins are a newly described family of proteins that may form gap junctions. We made antisera against mouse pannexin1 (Panx1). HeLa cells expressing Panx1 have cell surface labeling, but not gap junction plaques, and do not transfer small fluorescent dyes or neurobiotin in a scrape‐loading assay....

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Bibliographic Details
Published in:Glia 2007-01, Vol.55 (1), p.46-56
Main Authors: Huang, Yan, Grinspan, Judith B., Abrams, Charles K., Scherer, Steven S.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Antibody Specificity - immunology
astrocytes
Astrocytes - metabolism
Astrocytes - ultrastructure
Brain - cytology
Brain - growth & development
Cell Communication - physiology
Cell Differentiation - physiology
Cell Membrane - metabolism
Cell Membrane - ultrastructure
Cercopithecus aethiops
connexin
Connexins - genetics
Connexins - metabolism
COS Cells
Gap Junctions - metabolism
Gap Junctions - ultrastructure
HeLa Cells
Humans
Immunohistochemistry - methods
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neuroglia - metabolism
Neuroglia - ultrastructure
Neurons - metabolism
Neurons - ultrastructure
oligodendrocytes
Oligodendroglia - metabolism
Oligodendroglia - ultrastructure
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Summary:Pannexins are a newly described family of proteins that may form gap junctions. We made antisera against mouse pannexin1 (Panx1). HeLa cells expressing Panx1 have cell surface labeling, but not gap junction plaques, and do not transfer small fluorescent dyes or neurobiotin in a scrape‐loading assay. Neuro2a cells expressing Panx1 are not electrophysiologically coupled. Intracellular Panx1‐immunoreactivity, but not gap junction plaques, is seen in cultured oligodendrocytes, astrocytes, and hippocampal neurons. Thus, at least in these mammalian cells lines, Panx1 does not form morphological or functional gap junctions, and it remains to be demonstrated that Panx1 forms gap junction‐forming protein in the CNS. © 2006 Wiley‐Liss, Inc.
ISSN:0894-1491
1098-1136
DOI:10.1002/glia.20435