Quantitative assessment of small intraosseous prostate cancer burden in SCID mice using fluorescence imaging

BACKGROUND Experimental bone metastases are typically analyzed when the skeletal tumor burden is large enough to be detected by imaging or histology. By this time, the bone microenvironment is usually destroyed, preventing useful analysis of tumor–bone interactions. METHODS Small intraosseous tumors...

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Bibliographic Details
Published in:The Prostate 2007-01, Vol.67 (1), p.107-114
Main Authors: Yamamoto, Hamilto, Bonfil, R. Daniel, Wiesner, Christoph, Nabha, Sanaa, Dong, Zhong, Meng, Hong, Saliganan, Allen, Sabbota, Aaron, Chinni, Sreenivasa R., Cher, Michael L.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
bone metastasis
Bone Neoplasms - diagnostic imaging
Bone Neoplasms - pathology
Bone Neoplasms - secondary
Cell Line, Tumor
docetaxel
green fluorescent protein
Gynecology. Andrology. Obstetrics
Humans
Male
Male genital diseases
Medical sciences
Mice
Mice, SCID
Nephrology. Urinary tract diseases
prostate cancer
Prostatic Neoplasms - diagnostic imaging
Prostatic Neoplasms - pathology
Radiography
Spectrometry, X-Ray Emission - methods
Spectrometry, X-Ray Emission - standards
Tumors
Tumors of the urinary system
Urinary tract. Prostate gland
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Summary:BACKGROUND Experimental bone metastases are typically analyzed when the skeletal tumor burden is large enough to be detected by imaging or histology. By this time, the bone microenvironment is usually destroyed, preventing useful analysis of tumor–bone interactions. METHODS Small intraosseous tumors generated by intratibial injection of C4‐2B prostate cancer cells transfected with green fluorescent protein (GFP) were assessed using in vivo and ex vivo fluorescence imaging, radiography, histology, and fluorometric analysis of bone lysates. RESULTS Ex vivo fluorescence imaging and fluorometric analysis were capable of detecting tiny bone tumors as early as 10 days after injection. Ex vivo fluorescence imaging allowed simple quantification of small skeletal tumor burden and was useful in measuring the effect of systemic therapy. CONCLUSIONS Ex vivo fluorescence imaging is a sensitive and easy method to quantify small skeletal tumor burden. This technique allows investigation of tumor–bone interactions while the bone microanatomy is still intact. Prostate 67:107–114, 2007. © 2006 Wiley‐Liss, Inc.
ISSN:0270-4137
1097-0045
DOI:10.1002/pros.20506