effects of dietary phosphorus deficiency on surface pH and membrane composition of the mucosa epithelium in caprine jejunum

• Published in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Biochemical, systemic, and environmental physiology, 2007-01, Vol.177 (1), p.135-142
• Main Authors: Busche, R, Schröder, B, Huber, K, Sallmann, H. P, Breves, G
• Format: Article
• Language: English
• Subjects: Animals; Biological Transport - physiology; Cell Membrane - metabolism; Goats - metabolism; Hydrogen-Ion Concentration; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Jejunum - cytology; Jejunum - metabolism; Male; Membranes; Microvilli - metabolism; Phospholipids - metabolism; Phosphorus - deficiency; Phosphorus, Dietary - blood; Phosphorus, Dietary - pharmacology; Sodium-Hydrogen Exchangers - metabolism
• ISSN: 0174-1578; 1432-136X
• DOI: 10.1007/s00360-006-0118-3

In ruminants, the uptake of inorganic phosphate (Pi) across the intestinal mucosa epithelium by Na-dependent and Na-independent mechanisms is a main regulatory factor in P homeostasis. The aim of the study was to elucidate to which extent Na-independent mechanisms, including pH effects or composition of mucosal brush-border membranes, could be involved in positive stimulation of Pi absorptive processes seen under the P deficient condition. Therefore, luminal, surface and intracellular pH of the jejunal epithelial cells in control and P depleted goats were compared and biochemical analyses of membrane phospholipids in the apical membrane of the jejunal epithelium were performed. Dietary P depletion resulted in decreased plasma Pi levels. While pH in jejunal ingesta was not significantly changed, P depletion resulted in a significantly lower surface pH in the crypt region compared to control animals (7.62 ± 0.02 vs. 7.77 ± 0.04, n = 4, P < 0.01). Inhibition of apical Na⁺/H⁺-exchange resulted in an increase of the jejunal surface pH in P depleted animals by 0.07 ± 0.01 (n = 6, P < 0.01) and 0.05 ± 0.01 (n = 6, P < 0.01) for the villus and the crypt region, respectively. This increase were inversely correlated with the initial surface pH prior to inhibition. In contrast to surface pH, intracellular pH of the jejunal epithelium and the phospholipid composition of the apical jejunal membrane were not affected by P depletion. Although the data suggest the existence of a Na⁺/H⁺-exchange mechanism at the luminal surface of goat jejunum they do not support the hypothesis that adaptational processes of active Pi absorption from goat jejunum in response to low dietary P could be based on “non Pi transporter events”.