In Silico Identification and Mapping of Microsatellite Markers on Sus Scrofa Chromosome 4

Marker density of a QTL region on pig chromosome 4 was increased. New microsatellites were identified by in silico mining of BAC-end and genomic shotgun sequences. Among 8,784 BAC-end sequences predicted within the region, 148 microsatellites were identified. In addition, 27,450 CA/TG repeats were i...

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Bibliographic Details
Published in:Animal biotechnology 2007-01, Vol.18 (4), p.251-261
Main Authors: Wijk, H. J. van, Harlizius, B., Liefers, S. C., Buschbell, H., Dibbits, B., Groenen, M. A. M.
Format: Article
Language:English
Subjects:
Animals
carcass composition
Chromosome Mapping
Chromosomes, Artificial, Bacterial
Chromosomes, Mammalian
Computational Biology
Linkage map
Microsatellite marker
Microsatellite Repeats
Porcine
porcine genome
Quantitative Trait Loci
SSC4
SSR
Sus scrofa
Swine - genetics
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Summary:Marker density of a QTL region on pig chromosome 4 was increased. New microsatellites were identified by in silico mining of BAC-end and genomic shotgun sequences. Among 8,784 BAC-end sequences predicted within the region, 148 microsatellites were identified. In addition, 27,450 CA/TG repeats were identified within the genomic shotgun sequences, of which 157 were most likely located on SSC4q. A selection of 61 new microsatellites was mapped, together with previously mapped markers. The results showed that the human-pig comparative map in combination with BAC-end and genomic sequence resources provides an excellent source for a highly efficient and targeted development of markers.
ISSN:1049-5398
1532-2378
DOI:10.1080/10495390701399368