In Silico Identification and Mapping of Microsatellite Markers on Sus Scrofa Chromosome 4

Marker density of a QTL region on pig chromosome 4 was increased. New microsatellites were identified by in silico mining of BAC-end and genomic shotgun sequences. Among 8,784 BAC-end sequences predicted within the region, 148 microsatellites were identified. In addition, 27,450 CA/TG repeats were i...

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Published in:Animal biotechnology 2007-01, Vol.18 (4), p.251-261
Main Authors: Wijk, H. J. van, Harlizius, B., Liefers, S. C., Buschbell, H., Dibbits, B., Groenen, M. A. M.
Format: Article
Language:English
Subjects:
Animals
carcass composition
Chromosome Mapping
Chromosomes, Artificial, Bacterial
Chromosomes, Mammalian
Computational Biology
Linkage map
Microsatellite marker
Microsatellite Repeats
Porcine
porcine genome
Quantitative Trait Loci
SSC4
SSR
Sus scrofa
Swine - genetics
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cited_by cdi_FETCH-LOGICAL-c482t-3ef1afca33e2a0cf6c70b41138bf058ea65c2f75347606708185296ab2d6f7633
cites cdi_FETCH-LOGICAL-c482t-3ef1afca33e2a0cf6c70b41138bf058ea65c2f75347606708185296ab2d6f7633
container_end_page 261
container_issue 4
container_start_page 251
container_title Animal biotechnology
container_volume 18
creator Wijk, H. J. van
Harlizius, B.
Liefers, S. C.
Buschbell, H.
Dibbits, B.
Groenen, M. A. M.
description Marker density of a QTL region on pig chromosome 4 was increased. New microsatellites were identified by in silico mining of BAC-end and genomic shotgun sequences. Among 8,784 BAC-end sequences predicted within the region, 148 microsatellites were identified. In addition, 27,450 CA/TG repeats were identified within the genomic shotgun sequences, of which 157 were most likely located on SSC4q. A selection of 61 new microsatellites was mapped, together with previously mapped markers. The results showed that the human-pig comparative map in combination with BAC-end and genomic sequence resources provides an excellent source for a highly efficient and targeted development of markers.
doi_str_mv 10.1080/10495390701399368
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source Taylor and Francis Science and Technology Collection
subjects Animals
carcass composition
Chromosome Mapping
Chromosomes, Artificial, Bacterial
Chromosomes, Mammalian
Computational Biology
Linkage map
Microsatellite marker
Microsatellite Repeats
Porcine
porcine genome
Quantitative Trait Loci
SSC4
SSR
Sus scrofa
Swine - genetics
title In Silico Identification and Mapping of Microsatellite Markers on Sus Scrofa Chromosome 4
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