Clinical and Microbiological Profiles of Human Immunodeficiency Virus (HIV)–Seropositive Brazilians Undergoing Highly Active Antiretroviral Therapy and HIV‐Seronegative Brazilians With Chronic Periodontitis

• Published in: Journal of periodontology (1970) 2007-01, Vol.78 (1), p.87-96
• Main Authors: Gonçalves, Lucio de Souza, Soares Ferreira, Sônia Maria, Souza, Celso Oliveira, Souto, Renata, Colombo, Ana Paula
• Format: Article
• Language: English
• Subjects: Adult; Anti-HIV Agents - pharmacology; Antiretroviral Therapy, Highly Active; Bacteria, Anaerobic - drug effects; Bacteria, Anaerobic - isolation & purification; Biological and medical sciences; Case-Control Studies; Chronic Disease; Colony Count, Microbial; Dental Plaque - microbiology; Dentistry; DNA, Bacterial - analysis; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Female; Highly active antiretroviral therapy; HIV infection; HIV Seronegativity; HIV Seropositivity - complications; HIV Seropositivity - drug therapy; HIV Seropositivity - microbiology; Human viral diseases; Humans; Infectious diseases; Male; Medical sciences; Non tumoral diseases; Nucleic Acid Hybridization; Otorhinolaryngology. Stomatology; periodontitis; Periodontitis - complications; Periodontitis - microbiology; Statistics, Nonparametric; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1902/jop.2007.060040

Background: This study compares the periodontal clinical profile and the composition of the subgingival microbiota of human immunodeficiency virus (HIV)‐seropositive and HIV‐seronegative subjects with chronic periodontitis. Methods: A total of 172 subjects were distributed into two HIV‐seropositive groups (37 chronic periodontitis [H+CP+] and 35 periodontally healthy [H+CP−] individuals) and two HIV‐seronegative groups (49 chronic periodontitis [H−CP+] and 51 periodontally healthy [H−CP−] subjects). Subgingival samples were collected from six sites with the deepest probing depth in the periodontitis groups and six random sites in the groups with periodontal health. All HIV‐infected patients had undergone highly active antiretroviral therapy (HAART) for at least 2 years. The presence and levels of 33 bacterial species were detected by DNA probes and the checkerboard method. Kruskal‐Wallis and Mann‐Whitney tests were used to seek for significant differences among and between groups. Results: H−CP+ patients showed significantly more periodontal destruction and inflammation than H+CP+ patients, whereas H+CP− subjects presented a greater percentage of sites with bleeding than H−CP− subjects (P