Expression characteristics of prostate-derived Ets factor support a role in breast and prostate cancer progression

• Published in: Human pathology 2007-11, Vol.38 (11), p.1628-1638
• Main Authors: Sood, Ashwani K., PhD, Saxena, Rakhee, MD, Groth, Jeff, BS, Desouki, Mohamed M., MD, PhD, Cheewakriangkrai, Chalong, MD, Rodabaugh, Kerry J., MD, Kasyapa, Chitta S., PhD, Geradts, Joseph, MD
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Breast - metabolism; Breast cancer; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Breast Neoplasms - physiopathology; Cancer therapies; Cell Line, Tumor; Female; Gene Expression Regulation, Neoplastic; Gleason score; Gynecology. Andrology. Obstetrics; HeLa Cells; Humans; Investigative techniques, diagnostic techniques (general aspects); Male; Mammary gland diseases; Medical sciences; Metribolone - pharmacology; Mortality; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; PDEF; Prostate - metabolism; Prostate cancer; Prostatic Neoplasms - metabolism; Prostatic Neoplasms - pathology; Prostatic Neoplasms - physiopathology; Proteins; Proto-Oncogene Proteins c-ets - biosynthesis; Proto-Oncogene Proteins c-ets - immunology; R1881; Tumor marker and target; Tumors; U937 Cells
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/j.humpath.2007.03.010

Summary The purpose of this study was to understand the characteristics of prostate-derived Ets factor (PDEF) protein expression in breast and prostate cancer progression. A polyclonal antibody specific to PDEF was raised and reacted with tissue microarrays consisting of benign breast, in situ ductal, invasive ductal, and invasive lobular breast carcinomas. The antibody was also reacted with tissue microarrays, including benign prostate, prostate intraepithelial neoplasias (PINs), and prostate carcinomas. Increased expression of PDEF was identified in 18%, 50%, 46%, and 51% of benign breast tissues, intraductal, invasive ductal, and invasive lobular carcinomas, respectively. Importantly, in matched samples of benign breast vs tumor, 90% showed higher expression of PDEF in the tumor tissue. Moreover, in invasive breast carcinomas, increased PDEF expression tended to correlate with Her2/neu overexpression. Increased expression of PDEF was also found in 27%, 33%, and 40% of benign prostate tissues, PIN samples, and prostate adenocarcinomas, respectively. Again, in matching samples of cancer vs benign and cancer vs PIN, 68% and 70%, respectively, showed increased expression in the malignant tissue. Moreover, PDEF was found to be more highly expressed in tumors with intermediate or high Gleason score compared with low-grade tumors ( P < .01). In addition, R1881 treatment induced PDEF expression in the LNCaP prostate tumor cell line, suggesting regulation of PDEF by androgens in vivo. Together, these results for the first time show frequent increased expression of PDEF protein in breast and prostate tumors and support a role for PDEF in breast and prostate cancer progression.