The prevalence of chlamydiae of bulls from six bull studs in Germany

• Published in: Animal reproduction science 2007-11, Vol.102 (1), p.111-121
• Main Authors: Kauffold, Johannes, Henning, Klaus, Bachmann, Rüdiger, Hotzel, Helmut, Melzer, Falk
• Format: Article
• Language: English
• Subjects: Animals; antibody detection; bacterial infections; blood serum; breeding stock; Bull; bulls; Cattle; cattle diseases; Cattle Diseases - epidemiology; Chlamydia - classification; Chlamydia - isolation & purification; Chlamydia Infections - epidemiology; Chlamydia Infections - veterinary; Chlamydiae; Chlamydial antibodies; Chlamydophila abortus; Chlamydophila pecorum; Chlamydophila psittaci; disease detection; disease prevalence; disease transmission; epidemiological studies; Faeces; feces; Feces - microbiology; Germany - epidemiology; Male; male reproductive system; pathogen identification; pathogen shedding; Penis - microbiology; Preputial washing sample; Prevalence; risk assessment; Semen; Semen - microbiology; seroprevalence; sexually transmitted diseases
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/j.anireprosci.2006.10.013

Although there are indications for venereal transmission of chlamydiae in cattle, epidemiological data on the presence of these bacteria in bulls and bull semen in particular is still incomplete. We investigated semen ( n = 120), preputial washing samples ( n = 121) and faeces ( n = 122) of bulls from six bull studs located within five Federal States of Germany for the presence of chlamydiae using omp1-PCR and partial omp1 sequencing. Blood serum was examined for chlamydial antibodies using an indirect ELISA ( n = 122). Chlamydiae were found in 11 (9.2%), 13 (10.7%) and 22 (18.0%) of the semen, preputial washing and faecal samples, respectively. Among individual chlamydial species identified, Chlamydophila ( Cp.) psittaci predominated in semen and preputial washing samples, and Cp. pecorum in faeces. Cp. abortus was the third frequently observed species. Chlamydial antibodies were detected in a total of 62 (50.8%) bulls. Bull studs differed in regard to the number of bulls found chlamydia-positive in faeces and serologically positive. No correlation was observed between serological data and PCR of semen, preputial washing samples or faeces. Standard ejaculate parameters did not differ between bulls that were chlamydia-positive and -negative in semen. In conclusion, detection of chlamydiae in semen of bulls suggests a potential for venereal transmission. Chlamydiae appear to be widespread within the bull population in Germany. Serological testing failed to identify bulls shedding chlamydiae in their semen.