Effect of Probiotic Treatment in Broiler Chicks on Intestinal Macrophage Numbers and Phagocytosis of Salmonella Enteritidis by Abdominal Exudate Cells

• Published in: Poultry science 2007-11, Vol.86 (11), p.2315-2321
• Main Authors: Higgins, S.E, Erf, G.F, Higgins, J.P, Henderson, S.N, Wolfenden, A.D, Gaona-Ramirez, G, Hargis, B.M
• Format: Article
• Language: English
• Subjects: Abdomen; abdominal exudate cells; Animal Feed; Animal Nutritional Physiological Phenomena; animal pathogenic bacteria; Animals; broiler chickens; broiler feeding; cecum; Chickens; chicks; cultured cells; Diet - veterinary; Dietary Supplements; digesta; Exudates and Transudates - cytology; ileum; immune response; immunohistochemistry; intestinal mucosa; Intestines - cytology; Intestines - drug effects; Lactobacillus; macrophages; Macrophages - drug effects; phagocytosis; Phagocytosis - drug effects; Poultry Diseases - microbiology; Poultry Diseases - prevention & control; probiotics; Probiotics - pharmacology; Salmonella enteritidis; Salmonella Infections, Animal - prevention & control; temporal variation
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps.2007-00123

Previous data have indicated that a Lactobacillus-based probiotic culture (FM-B11) is efficacious in reducing Salmonella Enteritidis colonization within 24 h when administered within 1 h of challenge. We hypothesized that the innate immune system, specifically macrophages, may play a role in the observed reduction of Salmonella Enteritidis colonization with probiotic treatment. Day-of-hatch chicks were challenged with Salmonella Enteritidis and then treated with the probiotic culture 1 h later. Three other treatment groups were not treated (negative control), challenged only, or treated with probiotic only. In all experiments, probiotic treatment on the day of hatch reduced (P < 0.05) cecal Salmonella Enteritidis recovery as compared with the control treatment. In experiments 1 and 2, immunohistochemistry was used to evaluate the presence of macrophages (KUL01+) in the ileum and cecum of 7 to 10 chicks per group at 24 h posttreatment. In experiment 1, the number of macrophages observed per 10,000 μm² in the ileum of Salmonella Enteritidis-challenged chicks was higher (P < 0.05) than that of nonchallenged chicks (4.87 ± 0.31 vs. 3.05 ± 0.19). In the cecum, there were more (P < 0.05) macrophages per 10,000 μm² in chicks receiving probiotic treatment without challenge than in negative control chicks (5.32 ± 0.41 vs. 3.66 ± 0.35). However, in experiment 2 we found no differences among treatments in the numbers of macrophages for both the ileum and cecum. Experiments 3 and 4 were performed to evaluate the ability of Sephadex-elicited abdominal exudate cells (AEC) from chicks to phagocytose Salmonella Enteritidis in vitro. Abdominal exudate cells were isolated from the abdominal cavity, maintained in tissue culture plates overnight, and then assayed for phagocytic activity by coincubating with Salmonella Enteritidis. In experiment 3, more (P < 0.05) Salmonella Enteritidis was recovered from AEC derived from probiotic-treated chicks than in any other treatment. However, in experiment 4, all treatments resulted in similar levels of elicited AEC, and phagocytosis of Salmonella Enteritidis was at low levels in all groups. Although not conclusive, the modest differences detected in experiments 1 and 3, and the fact that those differences were not repeatedly detectable, suggest that these macrophage-related changes were not solely responsible for the reductions of Salmonella Enteritidis following probiotic treatment.