Microsatellite Status and Immunohistochemical Features of Ovarian Clear-cell Carcinoma

• Published in: Anticancer research 2005-07, Vol.25 (4), p.2785-2788
• Main Authors: UEDA, Haruhiko, WATANABE, Yoh, NAKAI, Hidekatsu, HEMMI, Hiromichi, KOI, Minoru, HOSHIAI, Hiroshi
• Format: Article
• Language: English
• Subjects: Adaptor Proteins, Signal Transducing; Adenocarcinoma, Clear Cell - genetics; Adenocarcinoma, Clear Cell - metabolism; Adult; Aged; Aged, 80 and over; Biological and medical sciences; Carrier Proteins; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Female; Female genital diseases; Genomic Instability; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry; Medical sciences; Microsatellite Repeats - genetics; Middle Aged; MutL Protein Homolog 1; MutS Homolog 2 Protein; MutS Homolog 3 Protein; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Ovarian Neoplasms - genetics; Ovarian Neoplasms - metabolism; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; Tumors
• ISSN: 0250-7005; 1791-7530

Background: Ovarian clear-cell carcinoma (OCC) is known to have a poor prognosis and selected genetic features of OCC remain unknown. We investigated microsatellite instability (MSI) and the expression of the DNA mismatch repair-related protein, p53. Materials and Methods: MSI was examined by polymerase chain reaction using mono-, di-, tri- and tetranucleotide repeat markers, and hMSH2, hMLH1, hMSH6, MSH3 and p53 were determined immunohistochemically in 24 cases of OCC. Results: A total of 9 (37.5%) cases exhibited MSI. Two cases (8.3%) exhibited MSI-H in mononucleotide repeat loci with the negative expression of hMLH1, while another 7 cases (29.2%) exhibited selected trinucloetide repeat MSI (MSI-TR). Of these MSI-TR cases, 4 cases (57.1%) were determined to be negative for MSH3, while hMSH2, hMSH6, MSH3 and p53 expressions were normal. Conclusion: Our findings suggest that MSI-TR would be a feature indicating the microsatellite status in OCC, and that the loss of MSH3 expression may promote MSI-TR.