Accumulation of S-adenosylmethionine induced oligopeptide transporters including BldK to regulate differentiation events in Streptomyces coelicolor M145

S-Adenosylmethionine (SAM), the major methyl donor in diverse biological processes, was recently found to be involved in the regulation of differentiation in streptomycetes. Exogenous SAM, in a quantity as low as 2 μM, enhanced antibiotic production and inhibited morphological development of Strepto...

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Bibliographic Details
Published in:FEMS microbiology letters 2005-08, Vol.249 (2), p.199-206
Main Authors: Park, Hyun-Suh, Shin, Su-Kyoung, Yang, Young-Yell, Kwon, Hyung-Jin, Suh, Joo-Won
Format: Article
Language:English
Subjects:
Adenosylmethionine
Antibiotics
Bacterial Proteins - metabolism
Bacteriology
Biological activity
Biological and medical sciences
BldK
Differentiation
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Genetics
Kinetics
Membrane Transport Proteins - metabolism
Microbiology
Microscopy, Electron, Scanning
Oligopeptides - metabolism
Permeability, membrane transport, intracellular transport
Regulation of differentiation
S-Adenosylmethionine
S-Adenosylmethionine - pharmacology
Streptomyces
Streptomyces coelicolor
Streptomyces coelicolor - drug effects
Streptomyces coelicolor - metabolism
Streptomyces coelicolor - ultrastructure
Streptomycetes
Two-dimensional gel electrophoresis
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Summary:S-Adenosylmethionine (SAM), the major methyl donor in diverse biological processes, was recently found to be involved in the regulation of differentiation in streptomycetes. Exogenous SAM, in a quantity as low as 2 μM, enhanced antibiotic production and inhibited morphological development of Streptomyces coelicolor M145. Total protein profiling of S. coelicolor M145 revealed that SAM enhanced the expression of oligopeptide-binding components related to ABC transporters that included BldK, a well-known regulatory factor in S. coelicolor differentiation. A radiolabeled SAM feeding experiment verified that exogenous SAM can be imported into the cell, which is under the control of the bld cascade. This study substantiated that BldK serves as a transducer of the SAM signal and uncovered the possible role of oligopeptide import in the regulation of Streptomyces differentiation, particularly in relation to SAM.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2005.05.047