Profiles of NK, NKT cell activation and cytokine production following vaccination against hepatitis B

Human natural killer (NK) cells (CD56+ CD3−) represent crucial components of the innate immune system especially against viral infections and because their activation can modulate the outcome of the adaptive immune response. NKT cells (CD56+CD3+), a lymphocyte T population characterized by expressio...

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Bibliographic Details
Published in:APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2005-07, Vol.113 (7-8), p.526-535
Main Authors: ALBARRAN, B., GONCALVES, L., SALMEN, S., BORGES, L., FIELDS, H., SOYANO, A., MONTES, H., BERRUETA, L.
Format: Article
Language:English
Subjects:
activation
Adult
Biological and medical sciences
CD56 Antigen - metabolism
cytokines
Cytokines - biosynthesis
Fundamental and applied biological sciences. Psychology
Hepatitis B - immunology
Hepatitis B - prevention & control
Hepatitis B Surface Antigens - administration & dosage
Hepatitis B Vaccines - administration & dosage
hepatitis B virus
Human viral diseases
Humans
Immunity, Innate
Infectious diseases
Innate immunity
Interferon-gamma - biosynthesis
Interleukin-2 - biosynthesis
Killer Cells, Natural - immunology
Lymphocyte Activation
Medical sciences
Microbiology
Middle Aged
Miscellaneous
NKT
T-Lymphocyte Subsets - immunology
Vaccines, Synthetic - administration & dosage
Viral diseases
Viral hepatitis
Virology
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Summary:Human natural killer (NK) cells (CD56+ CD3−) represent crucial components of the innate immune system especially against viral infections and because their activation can modulate the outcome of the adaptive immune response. NKT cells (CD56+CD3+), a lymphocyte T population characterized by expression of surface markers of NK cells, are known to be abundant in the liver and their activation could be associated with hepatic injury. Using three‐color flow cytometry to measure surface receptors and intracellular cytokines, we have explored early activation signals and cytokine production in NK and NKT cells within a group of hepatitis B vaccinated and non‐vaccinated individuals. A specific increase of the CD56bright cell population, the activation receptor CD69 and IFN‐γ, was observed in NK cells following incubation with recombinant HBsAg in responders to vaccination. Comparable results were observed in NKT cells showing an increment of CD69, CD25, IL‐2 and IFN‐γ expression in responder subjects. These parameters were statistically diminished in non‐responder individuals (p
ISSN:0903-4641
1600-0463
DOI:10.1111/j.1600-0463.2005.apm_191.x