Expression Profiles of Apoptotic Genes Induced by Curcumin in Human Breast Cancer and Mammary Epithelial Cell Lines

Curcumin (diferuloyl methane), the yellow-colored dietary pigment from the rhizomes of turmeric, has been recognized as a chemopreventive agent because of its antitumor, antioxidant and antiproliferative effects. The cytotoxic, apoptotic and gene regulatory effects of both turmeric and curcumin were...

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Bibliographic Details
Published in:Anticancer research 2005-09, Vol.25 (5), p.3293-3302
Main Authors: RAMACHANDRAN, Cheppail, RODRIGUEZ, Sonia, RAMACHANDRAN, Reshma, RAVEENDRAN NAIR, P. K, FONSECA, Hugo, KHATIB, Ziad, ESCALON, Enrique, MELNICK, Steven J
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
Apoptosis - drug effects
Apoptosis - genetics
Biological and medical sciences
Breast Neoplasms - drug therapy
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Cell Line, Tumor
Curcuma
Curcumin - pharmacology
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - physiology
Gene Expression Profiling
Gene Expression Regulation, Neoplastic - drug effects
Humans
Mammary Glands, Human - cytology
Mammary Glands, Human - drug effects
Mammary Glands, Human - physiology
Medical sciences
Oligonucleotide Array Sequence Analysis
Phytotherapy
Tumors
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Summary:Curcumin (diferuloyl methane), the yellow-colored dietary pigment from the rhizomes of turmeric, has been recognized as a chemopreventive agent because of its antitumor, antioxidant and antiproliferative effects. The cytotoxic, apoptotic and gene regulatory effects of both turmeric and curcumin were investigated in the MCF-7 human breast cancer carcinoma cell line and compared with the effects in MCF-10A human mammary epithelial cells. MCF-7 cells were more sensitive to turmeric and curcumin than MCF-10A cells. MCF-10A cells retained comparatively less curcumin in the medium than MCF-7 cells after 24 h, thereby reducing the cytotoxic effect. Curcumin induced a significantly higher percentage of apoptosis in MCF-7 than MCF-10A cells at all doses. Microarray hybridization of Clonetech apoptotic arrays with labeled first-strand probes of total RNA was performed to identify and characterize the genes regulated by curcumin in tumor cells. Of the 214 apoptosis-associated genes in the array, the expression of 104 genes was altered by curcumin treatment. The gene expression was altered up to14-fold levels in MCF-7 as compared to only up to 1.5-fold in the MCF-10A cell line by curcumin. Curcumin up-regulated (>3 fold) 22 genes and down-regulated (
ISSN:0250-7005
1791-7530