Intracrystalline proteins and urolithiasis: a comparison of the protein content and ultrastructure of urinary calcium oxalate monohydrate and dihydrate crystals

OBJECTIVE To compare the ultrastructure and protein content, particularly prothrombin fragment 1 and osteopontin, of calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) crystals precipitated from human urine, and their susceptibility to proteolysis, to try to clarify the role of in...

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Bibliographic Details
Published in:BJU international 2005-09, Vol.96 (4), p.654-663
Main Authors: Ryall, Rosemary L., Chauvet, Magali C., Grover, Phulwinder K.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Western - methods
calcium oxalate
Calcium Oxalate - chemistry
Calcium Oxalate - urine
Cathepsin D - chemistry
Crystallization
Electrophoresis, Polyacrylamide Gel
Endopeptidase K - chemistry
Female
Humans
intracrystalline proteins
Male
Medical sciences
Microscopy, Electron, Scanning
Nephrology. Urinary tract diseases
Osteopontin
Peptide Fragments - analysis
Protein Conformation
Protein Precursors - analysis
Prothrombin - analysis
prothrombin fragment 1
Sialoglycoproteins - analysis
Ultrafiltration
Urinary Calculi - metabolism
Urinary Calculi - urine
Urinary lithiasis
urine
urolithiasis
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Summary:OBJECTIVE To compare the ultrastructure and protein content, particularly prothrombin fragment 1 and osteopontin, of calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD) crystals precipitated from human urine, and their susceptibility to proteolysis, to try to clarify the role of intracrystalline proteins in urolithiasis, as differences between these types of crystal may determine whether calcium oxalate crystals nucleated in urine progress to stone formation. MATERIALS AND METHODS Sodium dodecyl sulphate gel electrophoresis and Western blotting were used to analyse demineralized extracts of COM and/or COD crystals deposited from the same centrifuged and filtered urine (which contains abundant urinary proteins) by adjusting the calcium concentration to 2 and 7 mmol/L, respectively. Similar analyses were performed on COM and COD crystals deposited from ultrafiltered urine (which contains only proteins of
ISSN:1464-4096
1464-410X
DOI:10.1111/j.1464-410X.2005.05701.x