Theory for the impact of basal turnover on dopamine clearance kinetics in the rat striatum after medial forebrain bundle stimulation and pressure ejection

Although microdialysis measurements suggest that extracellular dopamine concentrations in the rat striatum are in the low nanomolar range, some recent voltammetry studies suggest that the concentration may be considerably higher, perhaps in the micromolar range. The presence of such high dopamine le...

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Published in:Journal of neurochemistry 2005-09, Vol.94 (5), p.1202-1211
Main Authors: Michael, Adrian C., Borland, Laura M., J. Mitala, Joseph, Willoughby, Bridget M., Motzko, Christina M.
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description Although microdialysis measurements suggest that extracellular dopamine concentrations in the rat striatum are in the low nanomolar range, some recent voltammetry studies suggest that the concentration may be considerably higher, perhaps in the micromolar range. The presence of such high dopamine levels in the extracellular space has to be rationalized with the rapid, linear clearance of extracellular dopamine observed after electrical stimulation of the medial forebrain bundle. Kinetic analysis of dopamine clearance after evoked release suggests that the basal extracellular dopamine concentration is below the KM of dopamine uptake, which is near 0.2 µm. However, dopamine clearance after pressure ejection of dopamine into the rat striatum is slow and non‐linear, which may alternatively be a sign that basal dopamine release is only slightly slower than the maximal velocity of dopamine uptake, Vmax. A high basal extracellular dopamine concentration would exist if basal dopamine release were only slightly slower than the Vmax of uptake. This report introduces a new kinetic analysis of dopamine uptake that sheds light on the possible source of the different clearance rates observed following evoked dopamine release and dopamine pressure ejection. Furthermore, the analysis rationalizes the rapid dopamine clearance after evoked release with the possibility that basal extracellular dopamine levels are above the KM of the transporter.
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source Wiley:Jisc Collections:Wiley Read and Publish Open Access 2024-2025 (reading list); Free Full-Text Journals in Chemistry
subjects Animals
Biochemistry
Biological and medical sciences
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
Corpus Striatum - metabolism
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Dopamine - administration & dosage
Dopamine - metabolism
Dopamine - pharmacokinetics
dopamine uptake
Electric Stimulation
evoked release
Extracellular Space - metabolism
Fundamental and applied biological sciences. Psychology
Injections, Jet
kinetic model
Kinetics
Medial Forebrain Bundle - physiology
Medical sciences
Models, Neurological
Neurology
Neurotransmitters
pressure ejection
Rats
Rodents
striatum
Time Factors
Vertebrates: nervous system and sense organs
voltammetry
title Theory for the impact of basal turnover on dopamine clearance kinetics in the rat striatum after medial forebrain bundle stimulation and pressure ejection
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