Palmitic acid-induced activation of human T-lymphocytes and aortic endothelial cells with production of insulin receptors, reactive oxygen species, cytokines, and lipid peroxidation

Diabetic conditions are associated with hyperglycemia and hyperlipidemia, but the role of saturated fatty acids (SFA) vs. unsaturated fatty acids (UFA) in activation of T-lymphocytes and human aortic endothelial cells (HAEC) is not known. We investigated in vitro effects of various concentrations of...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-08, Vol.346 (3), p.721-726
Main Authors: Stentz, Frankie B., Kitabchi, Abbas E.
Format: Article
Language:English
Subjects:
Aorta - cytology
Cells, Cultured
Cytokines
Cytokines - metabolism
Endothelial Cells - cytology
Endothelial Cells - drug effects
Endothelial Cells - metabolism
Gene Expression Regulation
Human endothelial cells
Humans
Insulin receptor
Linoleic Acid - pharmacology
Lipid Peroxidation - drug effects
Lymphocyte Activation - drug effects
Oxidative stress
Palmitic acid
Palmitic Acid - pharmacology
Reactive Oxygen Species - metabolism
Receptor, Insulin - biosynthesis
T-lymphocytes
T-Lymphocytes - drug effects
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Unsaturated fatty acids
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Summary:Diabetic conditions are associated with hyperglycemia and hyperlipidemia, but the role of saturated fatty acids (SFA) vs. unsaturated fatty acids (UFA) in activation of T-lymphocytes and human aortic endothelial cells (HAEC) is not known. We investigated in vitro effects of various concentrations of SFA (palmitate) and UFA (oleic, linoleic, linolenic, and arachidonic) acids in activation of these cells. These cells in presence of palmitate, but not UFA, exhibited time, and concentration-dependent emergence of insulin receptors, GLUT 4 expression, generation of ROS, cytokines, lipid peroxidation, and IRS-1. We conclude that both T-lymphocytes and HAEC share common characteristics in exhibiting activation of these cells to palmitate, but not to UFA, by developing insulin receptors and becoming insulin responsive tissues, a hitherto unknown response to palmitate. We hypothesize that these events may serve as protective defense mechanisms against acute effects of glucotoxicity and lipotoxicity in these cells.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2006.05.159