Capping Transmembrane Helices of MscL with Aromatic Residues Changes Channel Response to Membrane Stretch

• Published in: Biochemistry (Easton) 2005-09, Vol.44 (37), p.12589-12597
• Main Authors: Chiang, Chien-Sung, Shirinian, Lena, Sukharev, Sergei
• Format: Article
• Language: English
• Subjects: Amino Acid Substitution; Escherichia coli - physiology; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - physiology; Ion Channel Gating - physiology; Ion Channels - chemistry; Ion Channels - physiology; Models, Molecular; Protein Structure, Secondary; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism; Stress, Mechanical; Tryptophan; Tyrosine
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi050750r

Tyrosines and tryptophans that anchor both ends of the helices to membrane interfaces in many transmembrane proteins are not common in MscL and homologous mechanosensitive channels. This characteristic absence of two aromatic “belts” may be critical for MscL function as the opening transition is predicted to be associated with a strong helical reorientation. A single tyrosine (Y75) on the extracellular side of the M2 helix of pentameric EcoMscL is absent in TbMscL, which instead has a single tyrosine (Y87) on the cytoplasmic side of M2. Moving the tyrosine of EcoMscL to the intracellular side (Y75F/F93Y) or capping the TM2 helix on both sides (F93Y/W) slows the kinetics of gating and increases the threshold for activation, leading to a partial loss-of-function in osmotic shock survival assays. Increasing the distance between the caps (L98W, L102Y/W) partially restores channel function presumably by loosening restraints for tilting. Capping the TM2 helix with a charged residue (Y75E) causes a right shift of the activation curve (“stiff” phenotype) and abolishes function. Introducing a “cap” into the TM1 helix (I41W) decreases the activation threshold and shortens the mean open time but unexpectedly leads to a complete loss-of-function in vivo. The data are consistent with the view that restraining helical positions in MscL by introducing specific protein−lipid interactions at membrane interfaces compromises MscL function. Subtle differences in osmotic shock survival are more evident at low levels of mutant protein expression. We observed a correlation between the right shift of tension activation threshold and the loss-of-function channel phenotype, with a few exceptions that point to other parameters of gating that may define the osmotic rescuing ability in vivo.