Evidence of Marginal-Zone B Cell- Positive Selection in Spleen

Antigen receptor-mediated signaling is critical for the development and survival of B cells. However, it has not been established whether B cell development requires a signal from self-ligand engagement at the immature stage, a process known as “positive selection.” Here, using a monoclonal B cell r...

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Bibliographic Details
Published in:Immunity (Cambridge, Mass.) Mass.), 2005-09, Vol.23 (3), p.297-308
Main Authors: Wen, Lijun, Brill-Dashoff, Joni, Shinton, Susan A., Asano, Masanao, Hardy, Richard R., Hayakawa, Kyoko
Format: Article
Language:English
Subjects:
Animals
Autoantibodies - immunology
Autoantigens - immunology
B-Lymphocytes - cytology
B-Lymphocytes - immunology
Bone marrow
Cell Differentiation - immunology
Cell Line
Clonal Deletion - immunology
Cloning
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fluorescent Antibody Technique
Immune system
Ligands
Lymphocytes
Mice
Mice, Transgenic
Receptor, Notch2
Receptors, Antigen, B-Cell - immunology
Receptors, Cell Surface - immunology
Receptors, Cell Surface - metabolism
Rodents
Spleen
Spleen - cytology
Spleen - immunology
T cell receptors
Thy-1 Antigens - immunology
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Summary:Antigen receptor-mediated signaling is critical for the development and survival of B cells. However, it has not been established whether B cell development requires a signal from self-ligand engagement at the immature stage, a process known as “positive selection.” Here, using a monoclonal B cell receptor (BCR) mouse line, specific for the self-Thy-1/CD90 glycoprotein, we demonstrate that BCR crosslinking by low-dose self-antigen promotes survival of immature B cells in culture. In spleen, an increase in BCR signaling strength, induced by low-dose self-antigen, directed naive immature B cells to mature, not into the default follicular B cell fate, but instead into the marginal-zone B cell subset. These data indicate that positive selection can occur in developing B cells and that BCR signal strength is a key factor in deciding between two functionally distinct mature B cell compartments in the microenvironment of the spleen.
ISSN:1074-7613
1097-4180
DOI:10.1016/j.immuni.2005.08.007