Cytoskeletal Coordination during Neuronal Migration

Discoveries from human and mouse genetics have identified cytoskeletal and signaling proteins that are essential for neuronal migration in the developing brain. To provide a meaningful context for these studies, we took an unbiased approach of correlative electron microscopy of neurons migrating thr...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2005-09, Vol.102 (38), p.13652-13657
Main Authors: Schaar, Bruce T., McConnell, Susan K., Tessier-Lavigne, Marc T.
Format: Article
Language:English
Subjects:
Animal migration behavior
Animals
Biological Sciences
Brain - embryology
Brain - ultrastructure
Cell adhesion & migration
Cell Adhesion - physiology
Cell membranes
Cell motility
Cell Movement - physiology
Cell nucleus
Cell Nucleus - physiology
Cell Nucleus - ultrastructure
Cells, Cultured
Centrosomes
Cytoskeleton
Humans
Imaging
Mice
Microscopy, Electron, Transmission
Microtubules
Microtubules - metabolism
Microtubules - ultrastructure
Myosin Type II - metabolism
Myosin Type II - ultrastructure
Neurology
Neurons
Neurons - physiology
Neurons - ultrastructure
Neutrophils
Nuclear membrane
Rats
Rats, Sprague-Dawley
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Summary:Discoveries from human and mouse genetics have identified cytoskeletal and signaling proteins that are essential for neuronal migration in the developing brain. To provide a meaningful context for these studies, we took an unbiased approach of correlative electron microscopy of neurons migrating through a three-dimensional matrix, and characterized the cytoskeletal events that occur as migrating neurons initiate saltatory forward movements of the cell nucleus. The formation of a cytoplasmic dilation in the proximal leading process precedes nuclear translocation. Cell nuclei translocate into these dilations in saltatory movements. Time-lapse imaging and pharmacological perturbation suggest that nucleokinesis requires stepwise or hierarchical interactions between microtubules, myosin II, and cell adhesion. We hypothesize that these interactions couple leading process extension to nuclear translocation during neuronal migration.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0506008102