Differential detergent fractionation for non-electrophoretic bovine peripheral blood monocyte proteomics reveals proteins involved in professional antigen presentation

• Published in: Developmental & Comparative Immunology 2006, Vol.30 (11), p.1070-1083
• Main Authors: Lee, S.-R., Pharr, G.T., Cooksey, A.M., McCarthy, F.M., Boyd, B.L., Pinchuk, L.M.
• Format: Article
• Language: English
• Subjects: Actin; Amino Acid Sequence; Animals; Antigen Presentation; Cattle - blood; Cattle - genetics; Cattle - immunology; Cell Adhesion Molecules - immunology; Cytokines; DDF; Dendritic Cells - immunology; Detergents - chemistry; Female; Gas Chromatography-Mass Spectrometry - veterinary; Growth factors; Histocompatibility Antigens Class I - genetics; Histocompatibility Antigens Class I - immunology; Histocompatibility Antigens Class II - genetics; Histocompatibility Antigens Class II - immunology; Lectin; Lectins, C-Type - immunology; Leukocytes, Mononuclear - chemistry; Leukocytes, Mononuclear - immunology; Lipopolysaccharide Receptors - analysis; Lipopolysaccharide Receptors - immunology; Mannose-Binding Lectins - immunology; MHC; Molecular Sequence Data; Monocytes; Progenitors; Proteomics - methods; Receptors, Cell Surface - immunology; Sequence Alignment; Spectrometry, Mass, Electrospray Ionization - veterinary; Subcellular Fractions - chemistry; TLR; Toll-Like Receptors - immunology
• ISSN: 0145-305X; 1879-0089; 1365-2567
• DOI: 10.1016/j.dci.2006.02.002

Professional antigen presenting cells (APC), dendritic cells (DC) and their myeloid progenitors, monocytes/macrophages are critical controllers of innate and adaptive immunity. Here we show that differential detergent fractionation (DDF) analysis of bovine monocytes reveals proteins related to antigen pattern recognition, uptake and presentation to immunocompetent lymphocytes. We identify 53 bovine proteins involved in immune function of professional APC. In particular, 13 adhesion molecules, three toll-like receptors (TLR1, 6 and 8), three antigen uptake-related proteins (including mannose receptor [MR] precursor), and eight actin-like proteins involved in active endocytosis were identified. In addition, MHC class I and II-related proteins, cytokines, active substances and growth factors have been identified. We conclude that the DDF approach can provide interpretable and meaningful functional information concerning protein expression profiles associated with monocyte activation, transformation into macrophages and/or immature DC, and maturation of monocyte-derived DC in the presence of multiple bovine pathogens.