Polyamine deficiency leads to accumulation of reactive oxygen species in a spe2Delta mutant of Saccharomyces cerevisiae

We have previously shown that polyamine-deficient Saccharomyces cerevisiae are very sensitive to incubation in oxygen. The current studies show that, even under more physiological conditions (i.e. growth in air), polyamine-deficient cells accumulate reactive oxygen species (ROS). These cells develop...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 2006-07, Vol.23 (10), p.751-761
Main Authors: Chattopadhyay, Manas K, Tabor, Celia White, Tabor, Herbert
Format: Article
Language:English
Subjects:
Apoptosis - physiology
Fluoresceins - pharmacology
Fluorescent Dyes - pharmacology
Microscopy, Confocal
Microscopy, Fluorescence
Mutation
Reactive Oxygen Species - metabolism
Rhodamines - pharmacology
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Spermidine - metabolism
Spermine - pharmacology
Superoxide Dismutase - metabolism
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Summary:We have previously shown that polyamine-deficient Saccharomyces cerevisiae are very sensitive to incubation in oxygen. The current studies show that, even under more physiological conditions (i.e. growth in air), polyamine-deficient cells accumulate reactive oxygen species (ROS). These cells develop an apoptotic phenotype and, after incubation in polyamine-deficient medium, die. To show a specific effect of polyamines on ROS accumulation, uncomplicated by any effects on growth, spermine was added to spermidine-deficient spe2Delta fms1Delta cells, since spermine does not affect the growth of this strain. In this strain, spermine addition caused a marked, but not complete, decrease in the accumulation of ROS and a moderate protection against cell death. In other experiments with polyamine-deficient cells containing plasmids that overexpress superoxide dismutases (SOD1, SOD2), ROS decreased but with only a partial protection against cell death. Polyamine-deficient cells incubated anaerobically show markedly less cell death. These data show that part of the function of polyamines is protection of the cells from accumulation of ROS.
ISSN:0749-503X