Mineralization of SaOS-2 cells on enzymatically (silicatein) modified bioactive osteoblast-stimulating surfaces

There is a demand for novel bioactive supports in surgery, orthopedics, and tissue engineering. The availability of recombinant silica‐synthesizing enzyme (silicatein) opens new possibilities for the synthesis of silica‐containing bioactive surfaces under ambient conditions that do not damage biomol...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2005-11, Vol.75B (2), p.387-392
Main Authors: Schröder, Heinz C., Boreiko, Oleksandra, Krasko, Anatoli, Reiber, Andreas, Schwertner, Heiko, Müller, Werner E. G.
Format: Article
Language:English
Subjects:
Biocompatible Materials - metabolism
Calcification, Physiologic - physiology
Calcium Phosphates - metabolism
Cathepsins - physiology
Cathepsins - ultrastructure
Cell Line, Tumor
collagen
Collagen Type I - physiology
Humans
hydroxyapatite
osteoblast mineralization
Osteoblasts - enzymology
Osteoblasts - physiology
Osteoblasts - ultrastructure
Recombinant Proteins - metabolism
Recombinant Proteins - ultrastructure
SaOS-2 cells
Silanes - metabolism
silica
silicatein
Substrate Specificity
Surface Properties
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Summary:There is a demand for novel bioactive supports in surgery, orthopedics, and tissue engineering. The availability of recombinant silica‐synthesizing enzyme (silicatein) opens new possibilities for the synthesis of silica‐containing bioactive surfaces under ambient conditions that do not damage biomolecules like proteins. Here it is shown that growth of human osteosarcoma SaOS‐2 cells on cluster plates precoated with Type 1 collagen is not affected by additional coating of the plates with the recombinant silicatein and incubation with its enzymatic substrate, tetraethoxysilane (TEOS). However, the enzymatic modification of the plates by biosilica deposition on the protein‐coated surface caused a marked increase in calcium phosphate formation of SaOS‐2 cells as revealed by alizarin red‐S staining to quantify calcium mineral content. The increased occurrence of calcium‐phosphate nodules on the modified surface was also observed by scanning electron microscopy. These results suggest that by supporting calcium‐phosphate deposition in vitro, biosilica (silicatein)‐modified surfaces are potentially bioactive in vivo, by stimulating osteoblast mineralization function. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2005
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.30322