Expression levels of the putative zinc transporter LIV‐1 are associated with a better outcome of breast cancer patients

• Published in: International journal of cancer 2005-12, Vol.117 (6), p.961-973
• Main Authors: Kasper, Grit, Weiser, Armin A., Rump, Andreas, Sparbier, Katrin, Dahl, Edgar, Hartmann, Arndt, Wild, Peter, Schwidetzky, Uta, Castaños‐Vélez, Esmeralda, Lehmann, Kerstin
• Format: Article
• Language: English
• Subjects: Base Sequence; Biological and medical sciences; Breast Neoplasms - chemistry; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cation Transport Proteins - analysis; Cation Transport Proteins - genetics; Disease-Free Survival; DNA, Complementary - chemistry; Drug Resistance, Neoplasm; Endoplasmic Reticulum - chemistry; estrogen receptor; Gene Expression; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry; In Situ Hybridization; LIV‐1; Mammary gland diseases; Medical sciences; Middle Aged; Molecular Sequence Data; Neoplasm Proteins - analysis; Neoplasm Proteins - genetics; Neoplasm Recurrence, Local; Prognosis; Receptors, Estrogen - analysis; RNA, Messenger - analysis; survival; Survival Rate; Tamoxifen; Tumors
• ISSN: 0020-7136; 1097-0215
• DOI: 10.1002/ijc.21235

We investigated the expression pattern of the breast cancer associated gene LIV‐1 on mRNA and protein level in 111 human breast cancer patients by in situ hybridization as well as immunohistochemistry and focused on the unknown potential of LIV‐1 expression levels as a prognostic marker. To our knowledge, this is the first study on endogenous LIV‐1 protein expression. Results of our study indicate that LIV‐1 mRNA and protein expression levels are only weakly correlated, suggesting posttranscriptional regulatory mechanisms. Furthermore, LIV‐1 mRNA quantity in combination with a positive ER status seem to represent a better marker than the progesterone receptor status according to the prognostic significance for relapse free survival (RFS). A negative correlation of LIV‐1 protein levels with tumor size, grade and stage reflects an association of LIV‐1 protein expression with less aggressive tumors. High LIV‐1 protein expression seems to be associated with a longer relapse free and overall survival in breast cancer patients with invasive ductal carcinoma. This association, however, seems to be dependent from other prognostic markers. Our data suggest that LIV‐1 is a promising candidate for a novel marker for breast cancer patients with better outcome. Furthermore, our study presents a revised cDNA sequence of LIV‐1 and demonstrates the localization of endogenous LIV‐1 in the endoplasmic reticulum. (Supplementary material for this article can be found on the International Journal of Cancer website at http://www.interscience. wiley.com/jpages/0020‐7136/suppmat/index.html). © 2005 Wiley‐Liss, Inc.