Increased association of Trypanosoma cruzi with sialoadhesin positive mice macrophages

Trypanosoma cruzi is a parasite with large amounts of sialic acid (SA) residues exposed at its surface that seems to be involved in macrophages infection. Some macrophages, present in T. cruzi infected tissues, expresses sialoadhesin (Sn), a receptor that recognizes SA. Thus, the involvement of Sn i...

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Published in:Parasitology research (1987) 2005-11, Vol.97 (5), p.380-385
Main Authors: MONTEIRO, Veronica G, LOBATO, Caroliny S. S, SILVA, Alexandre R, MEDINA, Diego V, DE OLIVEIRA, Marco A, SEABRA, Sergio H, DE SOUZA, Wanderley, DAMATTA, Renato A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cells, Cultured
Chagas Disease - parasitology
Erythrocytes - metabolism
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Invertebrates
Macrophages, Peritoneal - metabolism
Macrophages, Peritoneal - parasitology
Male
Membrane Glycoproteins - metabolism
Mice
Microscopy, Electron, Scanning
N-Acetylneuraminic Acid - metabolism
Receptors, Immunologic - metabolism
Sialic Acid Binding Ig-like Lectin 1
Trypanosoma cruzi
Trypanosoma cruzi - growth & development
Trypanosoma cruzi - pathogenicity
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Summary:Trypanosoma cruzi is a parasite with large amounts of sialic acid (SA) residues exposed at its surface that seems to be involved in macrophages infection. Some macrophages, present in T. cruzi infected tissues, expresses sialoadhesin (Sn), a receptor that recognizes SA. Thus, the involvement of Sn in the association of T. cruzi to macrophages was investigated. Sn was induced in mice peritoneal macrophages by homologous serum (HS) cultivation. Epimastigotes and trypomastigotes associated more to HS cultured macrophages than to fetal bovine serum (FBS). Blocking of Sn with antibodies reduced the association of trypomastigotes to similar level as for FBS cultured macrophages. Desialylation reduced the association of parasites to HS cultured macrophages indicating the Sn importance. Furthermore, the entrance mechanism of trypomastigotes to Sn positive macrophages has a phagocytic nature as demonstrated by scanning electron microscopy and cytochalasin D treatment. Sn positive macrophages may important in the initial trypomastigote infection, thus in the establishment of Chagas disease.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-005-1460-1