Material-Enhanced Laser Desorption/Ionization (MELDI)—A New Protein Profiling Tool Utilizing Specific Carrier Materials for Time of Flight Mass Spectrometric Analysis

Over the past couple of years, proteomics pattern analysis has emerged as an effective method for the early diagnosis of diseases such as ovarian, breast, or prostate cancer, without identification of single biomarkers. MALDI-TOF MS, for example, offers a simple approach for fast and reliable protei...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2006-09, Vol.17 (9), p.1203-1208
Main Authors: Feuerstein, Isabel, Najam-ul-Haq, Muhammad, Rainer, Matthias, Trojer, Lukas, Bakry, Rania, Aprilita, Nurul Hidayat, Stecher, Günther, Huck, Christian W., Bonn, Günther K., Klocker, Helmut, Bartsch, Georg, Guttman, Andras
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biological properties
Biomarkers
Chemical properties
Desorption
Diamonds
Divinylbenzene
Fingerprints
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Indicators and Reagents
Ionization
Ions
Lasers
Mass spectrometry
Pattern analysis
Peptide Mapping - methods
Prostate
Prostate cancer
Proteins
Proteins - chemistry
Proteomics
Sensitivity and Specificity
Silicon dioxide
Specimen Handling - methods
Spectrometry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:Over the past couple of years, proteomics pattern analysis has emerged as an effective method for the early diagnosis of diseases such as ovarian, breast, or prostate cancer, without identification of single biomarkers. MALDI-TOF MS, for example, offers a simple approach for fast and reliable protein profiling, especially by using carrier materials with various physical and chemical properties, in combination with a MALDI matrix. This approach is referred to as material-enhanced laser desorption/ionization (MELDI). In this paper, we report the development and application of derivatized carrier materials [cellulose, silica, poly(glycidyl methacrylate/divinylbenzene) (GMA/DVB) particles, and diamond powder] for fast and direct MALDI-TOF MS protein profiling. The applicability of MELDI for rapid protein profiling was evaluated with human serum samples. These carriers, having various hydrophobicities, resulted in characteristic mass fingerprints, even if all materials were derivatized with iminodiacetic acid (IDA) to yield an immobilized metal affinity chromatography (IMAC) functionality. Our study demonstrates that analyzing complex biological samples, such as human serum, by employing different MELDI carrier materials yielded type- and size-dependent performance variation.
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2006.04.032