Solid-phase extraction coupled with liquid chromatography–tandem mass spectrometry for determination of trace rosiglitazone in urine

This project evaluated solid-phase extraction (SPE) combined with liquid chromatography–tandem mass spectrometry (LC–MS/MS) to determine the trace amount of rosiglitazone in human urine. The analytical performance of four modes of LC–MS and tandem MS operation (atmospheric pressure chemical ionizati...

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Bibliographic Details
Published in:Journal of Chromatography A 2005-12, Vol.1097 (1), p.74-83
Main Authors: Chou, Chi-Chi, Lee, Maw-Rong, Cheng, Fu-Chou, Yang, Dar-Yu
Format: Article
Language:English
Subjects:
Administration, Oral
Analysis
Biological and medical sciences
Chemistry Techniques, Analytical - instrumentation
Chemistry Techniques, Analytical - methods
Chromatography, Liquid - methods
Female
General pharmacology
Humans
Hypoglycemic Agents - administration & dosage
Hypoglycemic Agents - urine
Liquid chromatography
Medical sciences
Pharmacology. Drug treatments
Reproducibility of Results
Rosiglitazone
Solid-phase extraction
Spectrometry, Mass, Electrospray Ionization - methods
Tandem mass spectrometry
Thiazolidinediones - administration & dosage
Thiazolidinediones - urine
Time Factors
Urine
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Summary:This project evaluated solid-phase extraction (SPE) combined with liquid chromatography–tandem mass spectrometry (LC–MS/MS) to determine the trace amount of rosiglitazone in human urine. The analytical performance of four modes of LC–MS and tandem MS operation (atmospheric pressure chemical ionization (APCI), electrospray ionization (ESI), positive and negative ionization) was compared for two mass spectrometers, a triple-quadrupole and a quadrupole ion trap instrument. Rosiglitazone was extracted from urine using a SPE cartridge of 50 mg C8 sorbent and acetonitrile used as the eluting solvent. Samples were then separated on a RP18 column interfaced with a tandem mass spectrometer. The recovery of rosiglitazone was greater than 91.2%. The urine assay combining SPE and LC–APCI-MS/MS of triple-quadrupole was proved a very selective and sensitive method for determination of trace rosiglitazone. The assay was linear over a wide range, with a lower limit of quantification of 0.1 ng/mL using 1 mL of urine. The intra- and inter-day precisions were
ISSN:0021-9673
DOI:10.1016/j.chroma.2005.08.033