Gene Expression of Ornithine Decarboxylase in Lung Cancers and Its Clinical Significance

Lung cancer is one of the most lethal cancers in China because of its high incidence and high mortality. Ornithine decarboxylase (ODC), an important enzyme in polyamine biosynthesis, is increased in cancer cells. Some chemotherapeutic agents aimed at reducing ODC expression show inhibitory effects o...

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Published in:Acta biochimica et biophysica Sinica 2006-09, Vol.38 (9), p.639-645
Main Authors: TIAN, Hui, HUANG, Qing, LI, Lin, LIU, Xian‐Xi, ZHANG, Yan
Format: Article
Language:English
Subjects:
Adenovirus
Animals
A‐549 cell line
Cytomegalovirus
Gene Expression - genetics
Gene Silencing
Gene Targeting
Genetic Therapy - methods
lung neoplasm
Lung Neoplasms - enzymology
Lung Neoplasms - genetics
Lung Neoplasms - therapy
Male
Mice
Mice, Inbred BALB C
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
ornithine decarboxylase
Ornithine Decarboxylase - genetics
Ornithine Decarboxylase - metabolism
polyamine biosynthesis
Treatment Outcome
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Summary:Lung cancer is one of the most lethal cancers in China because of its high incidence and high mortality. Ornithine decarboxylase (ODC), an important enzyme in polyamine biosynthesis, is increased in cancer cells. Some chemotherapeutic agents aimed at reducing ODC expression show inhibitory effects on cancer cell growth, so ODC can be useful in gene diagnosis and gene therapy of cancers. In this study, we examined the effect of antisense ODC on lung cancer cells. A‐549 cells were infected with rAd‐ODC/Ex3as, a recombinant adenovirus containing the cytomegalovirus promoter, green fluorescent protein gene and 120 bp antisense ODC. The cell cycle was evaluated by flow cytometry. A nude mouse xenograft model was used in the tumorigenicity test. Reverse transcription‐polymerase chain reaction, Western blot and immunohistochemistry were used to study the expressions of ODC on lung cancers. It was found that the growth of cells infected with rAd‐ODC/Ex3as was substantially inhibited and cells were arrested at G1 phase. Cells infected with rAd‐ODC/Ex3as can suppress tumor formation in a nude mouse xenograft model. The expression of ODC mRNA and ODC protein levels in lung cancer tissues was significantly higher than that in normal tissues (P
ISSN:1672-9145
1745-7270
DOI:10.1111/j.1745-7270.2006.00204.x