Absolute oxygen tension (pO2) in murine fatty and muscle tissue as determined by EPR

The absolute partial pressure of oxygen (pO2) in the mammary gland pad and femoral muscle of female mice was measured using EPR oximetry at 700 MHz. A small quantity of lithium phthalocyanine (LiPc) crystals was implanted in both mammary and femoral muscle tissue of female C3H mice. Subsequent EPR m...

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Published in:Magnetic resonance in medicine 2005-12, Vol.54 (6), p.1530-1535
Main Authors: Matsumoto, Atsuko, Matsumoto, Shingo, Sowers, Anastasia L., Koscielniak, Janusz W., Trigg, Nancy J., Kuppusamy, Periannan, Mitchell, James B., Subramanian, Sankaran, Krishna, Murali C., Matsumoto, Ken-ichiro
Format: Article
Language:English
Subjects:
Adipose Tissue - metabolism
Animals
electron paramagnetic resonance
Electron Spin Resonance Spectroscopy - methods
Female
Indoles
lithium phthalocyanine
mammary gland
Mice
Mice, Inbred C3H
mouse
Muscle, Skeletal - metabolism
Organometallic Compounds
Oxygen - metabolism
oxygen concentration
Oxygen Consumption - physiology
Reproducibility of Results
Sensitivity and Specificity
skeletal muscle
Spin Labels
spin probe
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Summary:The absolute partial pressure of oxygen (pO2) in the mammary gland pad and femoral muscle of female mice was measured using EPR oximetry at 700 MHz. A small quantity of lithium phthalocyanine (LiPc) crystals was implanted in both mammary and femoral muscle tissue of female C3H mice. Subsequent EPR measurements were carried out 1–30 days after implantation with or without control of core body temperature. The pO2 values in the tissue became stable 2 weeks after implantation of LiPc crystals. The pO2 level was found to be higher in the femoral muscle than in the mammary tissue. However, the pO2 values showed a strong dependence on the core body temperature of the mice. The pO2 values were responsive to carbogen (95% O2, 5% CO2) breathing even 44–58 days after the implantation of LiPc. The LiPc linewidth was also sensitive to changes in the blood supply even 60 days after implantation of the crystals. This study further validates the use of LiPc crystals and EPR oximetry for long‐term non‐invasive assessment of pO2 levels in tissues, underscores the importance of maintaining normal body core temperature during the measurements, and demonstrates that mammary tissue functions at a lower pO2 level than muscle in female C3H mice. Magn Reson Med, 2005. Published 2005 Wiley‐Liss, Inc.
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.20714